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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species
Author(s): Vallejo-Marin, Mario
Solis-Montero, Lislie
Bacles, Cecile
Lepais, Olivier
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Keywords: invasive species
population genetics
reproductive biology
Solanum rostratum
Solanum fructu-tecto
Solanum heterodoxum
Solanum grayi var. grayi
Solanum grayi var. grandiflorum
Solanum lumholtzianum
Solanum sect. Androceras
Issue Date: Oct-2011
Citation: Vallejo-Marin M, Solis-Montero L, Bacles C & Lepais O (2011) Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species, American Journal of Botany, 98 (10), pp. e296-e299.
Abstract: Premise of the study: Microsatellite markers were developed using second-generation sequencing in Solanum rostratum as a tool to study the reproductive biology and genetic structure of this invasive species. Methods and Results: Thirteen microsatellites were successfully discovered and amplified in a single multiplexed PCR. All loci showed genetic variation in S. rostratum. Cross-amplification in five closely related taxa was successful for a subset of loci. Conclusions: The set of 13 microsatellite markers developed here provides a time-effective and cost-effective genetic tool to study the reproductive biology of S. rostratum. The demonstrated transferability of the PCR multiplex to related taxa also highlights its usefulness for evolutionary studies across Solanum sect. Androceras.
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