Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/8750
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dc.contributor.authorVallejo-Marin, Mario-
dc.contributor.authorSolis-Montero, Lislie-
dc.contributor.authorBacles, Cecile-
dc.contributor.authorLepais, Olivier-
dc.date.accessioned2018-04-24T02:58:32Z-
dc.date.available2018-04-24T02:58:32Z-
dc.date.issued2011-10-
dc.identifier.urihttp://hdl.handle.net/1893/8750-
dc.description.abstractPremise of the study: Microsatellite markers were developed using second-generation sequencing in Solanum rostratum as a tool to study the reproductive biology and genetic structure of this invasive species. Methods and Results: Thirteen microsatellites were successfully discovered and amplified in a single multiplexed PCR. All loci showed genetic variation in S. rostratum. Cross-amplification in five closely related taxa was successful for a subset of loci. Conclusions: The set of 13 microsatellite markers developed here provides a time-effective and cost-effective genetic tool to study the reproductive biology of S. rostratum. The demonstrated transferability of the PCR multiplex to related taxa also highlights its usefulness for evolutionary studies across Solanum sect. Androceras.en_UK
dc.language.isoen-
dc.publisherBotanical Society of America-
dc.relationVallejo-Marin M, Solis-Montero L, Bacles C & Lepais O (2011) Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species, American Journal of Botany, 98 (10), pp. e296-e299.-
dc.rightsPublisher policy allows this work to be made available in this repository. Published in American Journal of Botany by Botanical Society of America . The original publication is available at http://www.amjbot.org/content/98/10/e296-
dc.subjectinvasive speciesen_UK
dc.subjectpopulation geneticsen_UK
dc.subjectreproductive biologyen_UK
dc.subjectSolanum rostratumen_UK
dc.subjectSolanum fructu-tectoen_UK
dc.subjectSolanum heterodoxumen_UK
dc.subjectSolanum grayi var. grayien_UK
dc.subjectSolanum grayi var. grandiflorumen_UK
dc.subjectSolanum lumholtzianumen_UK
dc.subjectSolanum sect. Androcerasen_UK
dc.titleThirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related speciesen_UK
dc.typeJournal Articleen_UK
dc.identifier.doihttp://dx.doi.org/10.3732/ajb.1100159-
dc.identifier.pmid21980166-
dc.citation.jtitleAmerican Journal of Botany-
dc.citation.issn0002-9122-
dc.citation.volume98-
dc.citation.issue10-
dc.citation.spagee296-
dc.citation.epagee299-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPost-print (author final draft post-refereeing)-
dc.author.emailmario.vallejo@stir.ac.uk-
dc.contributor.affiliationBiological and Environmental Sciences-
dc.contributor.affiliationUniversity of Stirling-
dc.contributor.affiliationBiological and Environmental Sciences-
dc.contributor.affiliationBiological and Environmental Sciences-
dc.identifier.isi000295888800012-
Appears in Collections:Biological and Environmental Sciences Journal Articles

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