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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Chitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariae
Author(s): Suginta, Wipa
Robertson, Peter
Austin, Brian
Fry, Stephen C
Fothergill-Gilmore, Linda A
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Issue Date: Jul-2000
Date Deposited: 8-Aug-2012
Citation: Suginta W, Robertson P, Austin B, Fry SC & Fothergill-Gilmore LA (2000) Chitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariae. Journal of Applied Microbiology, 89 (1), pp. 76-84.
Abstract: Fourteen species of Vibrio were screened for chitin-induced chitinase activity in culture medium. V. carchariae, V. alginolyticus 283 and V. campbellii showed high levels of activity. Screening on agar plates containing swollen chitin showed high levels of chitinase activity by the same three species, and also by V. fischeri and V. alginolyticus 284. An affinity purification procedure was developed for the chitinase from V. carchariae. The purified chitinase was active as a monomer with Mr 63 000–66 000, and displayed activity toward polymeric chitin from acetylated chitosan or from crab shells. N-terminal sequence analysis and immunological cross-reactivity confirmed that the enzyme belongs to the group A/chiA family of bacterial chitinases.
DOI Link: 10.1046/j.1365-2672.2000.01076.x
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