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dc.contributor.authorSuginta, Wipa-
dc.contributor.authorRobertson, Peter-
dc.contributor.authorAustin, Brian-
dc.contributor.authorFry, Stephen C-
dc.contributor.authorFothergill-Gilmore, Linda A-
dc.description.abstractFourteen species of Vibrio were screened for chitin-induced chitinase activity in culture medium. V. carchariae, V. alginolyticus 283 and V. campbellii showed high levels of activity. Screening on agar plates containing swollen chitin showed high levels of chitinase activity by the same three species, and also by V. fischeri and V. alginolyticus 284. An affinity purification procedure was developed for the chitinase from V. carchariae. The purified chitinase was active as a monomer with Mr 63 000–66 000, and displayed activity toward polymeric chitin from acetylated chitosan or from crab shells. N-terminal sequence analysis and immunological cross-reactivity confirmed that the enzyme belongs to the group A/chiA family of bacterial chitinaseen_UK
dc.relationSuginta W, Robertson P, Austin B, Fry SC & Fothergill-Gilmore LA (2000) Chitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariae, Journal of Applied Microbiology, 89 (1), pp. 76-84.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.titleChitinases from Vibrio: activity screening and purification of chiA from Vibrio carchariaeen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleJournal of Applied Microbiology-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationUniversity of Edinburgh-
dc.contributor.affiliationHeriot-Watt University-
dc.contributor.affiliationHeriot-Watt University-
dc.contributor.affiliationUniversity of Edinburgh-
dc.contributor.affiliationUniversity of Edinburgh-
Appears in Collections:Aquaculture Journal Articles

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