|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyi|
Morris, Peter C
|Citation:||Prasad S, Morris PC, Hansen R, Meaden P & Austin B (2005) A novel bacteriocin- like substance (BLIS) from a pathogenic strain of Vibrio harveyi, Microbiology, 151 (9), pp. 3051-3058.|
|Abstract:||Inter-strain and inter-species inhibition mediated by a bacteriocin-like inhibitory substance (BLIS) from a pathogenic Vibrio harveyi strain VIB 571 was demonstrated against four isolates of the same species, and one culture each of a Vibrio sp., Vibrio fischeri, Vibrio gazogenes and Vibrio parahaemolyticus. The crude BLIS, which was obtained by ammonium-sulphate precipitation of the cell- free supernatant of a 72 h broth culture of strain VIB 571, was inactivated by lipase, proteinase K, pepsin, trypsin, pronase E, SDS and incubation at ¢60 6C for 10 min. The activity was stable between pH 2-11 for at least 5 h. Anion- exchange chromatography, gel filtration, SDS-PAGE and two-dimensional gel electrophoresis revealed the presence of a single major peak, comprising a protein with a pI of ~5?4 and a molecular mass of ~32 kDa. The N-terminal amino acid sequence of the protein comprised Asp-Glu-Tyr-Ile-Ser-X-Asn-Lys-X-Ser-Ser- Ala-Asp-Ile (with X representing cysteine or modified amino acid residues). A similarity search based on the matrix-assisted laser desorption/ionization time- of-flight mass spectrometry (MALDI-TOF-MS) generated peptide masses and the N- terminal sequence did not yield any significant matches|
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