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dc.contributor.authorPrasad, Sathishen_UK
dc.contributor.authorMorris, Peter Cen_UK
dc.contributor.authorHansen, Rasmusen_UK
dc.contributor.authorMeaden, Philipen_UK
dc.contributor.authorAustin, Brianen_UK
dc.description.abstractInter-strain and inter-species inhibition mediated by a bacteriocin-like inhibitory substance (BLIS) from a pathogenic Vibrio harveyi strain VIB 571 was demonstrated against four isolates of the same species, and one culture each of a Vibrio sp., Vibrio fischeri, Vibrio gazogenes and Vibrio parahaemolyticus. The crude BLIS, which was obtained by ammonium-sulphate precipitation of the cell-free supernatant of a 72 h broth culture of strain VIB 571, was inactivated by lipase, proteinase K, pepsin, trypsin, pronase E, SDS and incubation at ¢60 6C for 10 min. The activity was stable between pH 2-11 for at least 5 h. Anion-exchange chromatography, gel filtration, SDS-PAGE and two-dimensional gel electrophoresis revealed the presence of a single major peak, comprising a protein with a pI of ~5?4 and a molecular mass of ~32 kDa. The N-terminal amino acid sequence of the protein comprised Asp-Glu-Tyr-Ile-Ser-X-Asn-Lys-X-Ser-Ser-Ala-Asp-Ile (with X representing cysteine or modified amino acid residues). A similarity search based on the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) generated peptide masses and the N-terminal sequence did not yield any significant matches.en_UK
dc.publisherSociety for General Microbiologyen_UK
dc.relationPrasad S, Morris PC, Hansen R, Meaden P & Austin B (2005) A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyi. Microbiology, 151 (9), pp. 3051-3058.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.titleA novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyien_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Austin_2005_A_novel_bacteriocin-like_substance.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationHeriot-Watt Universityen_UK
dc.contributor.affiliationHeriot-Watt Universityen_UK
dc.contributor.affiliationHeriot-Watt Universityen_UK
dc.contributor.affiliationHeriot-Watt Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorPrasad, Sathish|en_UK
local.rioxx.authorMorris, Peter C|en_UK
local.rioxx.authorHansen, Rasmus|en_UK
local.rioxx.authorMeaden, Philip|en_UK
local.rioxx.authorAustin, Brian|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Aquaculture Journal Articles

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