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http://hdl.handle.net/1893/32634| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Development and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatus |
| Author(s): | Lu, Junkai Li, Ronghua Bekaert, Michaël Migaud, Herve Liu, Xiao Chen, Qingwei Zhang, Weiren Mu, Changkao Song, Weiwei Wang, Chunlin |
| Contact Email: | michael.bekaert@stir.ac.uk |
| Keywords: | Sex-linked SNP Competitive allele-specific PCR assay KASP Portunus trituberculatus |
| Issue Date: | Jul-2021 |
| Date Deposited: | 25-May-2021 |
| Citation: | Lu J, Li R, Bekaert M, Migaud H, Liu X, Chen Q, Zhang W, Mu C, Song W & Wang C (2021) Development and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatus. Aquaculture Reports, 20, Art. No.: 100731. https://doi.org/10.1016/j.aqrep.2021.100731 |
| Abstract: | The swimming crab Portunus trituberculatus is an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference and enhanced growth performance. However, the lack of a rapid and reliable sex-linked marker panel has hampered the application of sex control and understanding of the sex determination in the species. In this study, two sex-linked SNPs obtained from our simplified genomic data were validated by sequencing and then converted into competitive allele-specific PCR assays. The results showed high consistency between the genetic and phenotypic sex providing therefore a rapid test sex identification. Moreover, heterogametic genotypes in males provided a significant genetic evidence to support an XX/XY sex determination system in the P. trituberculatus. |
| DOI Link: | 10.1016/j.aqrep.2021.100731 |
| Rights: | This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed. For commercial reuse, permission must be requested. |
| Licence URL(s): | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 1-s2.0-S2352513421001472-main.pdf | Fulltext - Published Version | 2.09 MB | Adobe PDF | View/Open |
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