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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/32634
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dc.contributor.authorLu, Junkaien_UK
dc.contributor.authorLi, Ronghuaen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorMigaud, Herveen_UK
dc.contributor.authorLiu, Xiaoen_UK
dc.contributor.authorChen, Qingweien_UK
dc.contributor.authorZhang, Weirenen_UK
dc.contributor.authorMu, Changkaoen_UK
dc.contributor.authorSong, Weiweien_UK
dc.contributor.authorWang, Chunlinen_UK
dc.date.accessioned2021-05-26T00:04:49Z-
dc.date.available2021-05-26T00:04:49Z-
dc.date.issued2021-07en_UK
dc.identifier.other100731en_UK
dc.identifier.urihttp://hdl.handle.net/1893/32634-
dc.description.abstractThe swimming crab Portunus trituberculatus is an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference and enhanced growth performance. However, the lack of a rapid and reliable sex-linked marker panel has hampered the application of sex control and understanding of the sex determination in the species. In this study, two sex-linked SNPs obtained from our simplified genomic data were validated by sequencing and then converted into competitive allele-specific PCR assays. The results showed high consistency between the genetic and phenotypic sex providing therefore a rapid test sex identification. Moreover, heterogametic genotypes in males provided a significant genetic evidence to support an XX/XY sex determination system in the P. trituberculatus.en_UK
dc.language.isoenen_UK
dc.publisherElsevier BVen_UK
dc.relationLu J, Li R, Bekaert M, Migaud H, Liu X, Chen Q, Zhang W, Mu C, Song W & Wang C (2021) Development and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatus. Aquaculture Reports, 20, Art. No.: 100731. https://doi.org/10.1016/j.aqrep.2021.100731en_UK
dc.rightsThis article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed. For commercial reuse, permission must be requested.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectSex-linked SNPen_UK
dc.subjectCompetitive allele-specific PCR assayen_UK
dc.subjectKASPen_UK
dc.subjectPortunus trituberculatusen_UK
dc.titleDevelopment and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatusen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.aqrep.2021.100731en_UK
dc.citation.jtitleAquaculture Reportsen_UK
dc.citation.issn2352-5134en_UK
dc.citation.volume20en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailmichael.bekaert@stir.ac.uken_UK
dc.citation.date24/05/2021en_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.identifier.isiWOS:000674482200011en_UK
dc.identifier.scopusid2-s2.0-85106364452en_UK
dc.identifier.wtid1730220en_UK
dc.contributor.orcid0000-0002-8282-3232en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.contributor.orcid0000-0002-5404-7512en_UK
dc.date.accepted2021-05-21en_UK
dcterms.dateAccepted2021-05-21en_UK
dc.date.filedepositdate2021-05-25en_UK
dc.subject.tagAquaculture: Geneticsen_UK
dc.subject.tagAquaculture: Genomicsen_UK
dc.subject.tagAquaculture Innovationen_UK
dc.subject.tagAquaculture: New Speciesen_UK
dc.subject.tagBioinformaticsen_UK
dc.subject.tagMarine Biotechnologyen_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorLu, Junkai|en_UK
local.rioxx.authorLi, Ronghua|0000-0002-8282-3232en_UK
local.rioxx.authorBekaert, Michaël|0000-0002-1206-7654en_UK
local.rioxx.authorMigaud, Herve|0000-0002-5404-7512en_UK
local.rioxx.authorLiu, Xiao|en_UK
local.rioxx.authorChen, Qingwei|en_UK
local.rioxx.authorZhang, Weiren|en_UK
local.rioxx.authorMu, Changkao|en_UK
local.rioxx.authorSong, Weiwei|en_UK
local.rioxx.authorWang, Chunlin|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2021-05-25en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2021-05-25|en_UK
local.rioxx.filename1-s2.0-S2352513421001472-main.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source2352-5134en_UK
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