Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31504
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Dietary starch promotes hepatic lipogenesis in barramundi (Lates calcarifer)
Author(s): Wade, Nicholas M
Trenkner, Lauren H
Viegas, Ivan
Tavares, Ludgero C
Palma, Mariana
Skiba-Cassy, Sandrine
Dias, Karine
Vachot, Christiane
Araujo, Bruno C
Bourne, Nicholas
Blyth, David
Irvin, Simon
Glencross, Brett D
Contact Email: b.d.glencross@stir.ac.uk
Keywords: Liver
Fish
Nutrigenomics
2H
Lipogenic flux
NMR
Issue Date: 28-Aug-2020
Citation: Wade NM, Trenkner LH, Viegas I, Tavares LC, Palma M, Skiba-Cassy S, Dias K, Vachot C, Araujo BC, Bourne N, Blyth D, Irvin S & Glencross BD (2020) Dietary starch promotes hepatic lipogenesis in barramundi (Lates calcarifer). British Journal of Nutrition, 124 (4), pp. 363-373. https://doi.org/10.1017/s0007114520001051
Abstract: Barramundi (Lates calcarifer) are a highly valued aquaculture species, and, as obligate carnivores, they have a demonstrated preference for dietary protein over lipid or starch to fuel energetic growth demands. In order to investigate how carnivorous fish regulate nutritional cues, we examined the metabolic effects of feeding two isoenergetic diets that contained different proportions of digestible protein or starch energy. Fish fed a high proportion of dietary starch energy had a higher proportion of liver SFA, but showed no change in plasma glucose levels, and few changes in the expression of genes regulating key hepatic metabolic pathways. Decreased activation of the mammalian target of rapamycin growth signalling cascade was consistent with decreased growth performance values. The fractional synthetic rate (lipogenesis), measured by TAG 2H-enrichment using 2H NMR, was significantly higher in barramundi fed with the starch diet compared with the protein diet (0·6 (se 0·1) v. 0·4 (se 0·1) % per d, respectively). Hepatic TAG-bound glycerol synthetic rates were much higher than other closely related fish such as sea bass, but were not significantly different (starch, 2·8 (se 0·3) v. protein, 3·4 (se 0·3) % per d), highlighting the role of glycerol as a metabolic intermediary and high TAG-FA cycling in barramundi. Overall, dietary starch significantly increased hepatic TAG through increased lipogenesis. Compared with other fish, barramundi possess a unique mechanism to metabolise dietary carbohydrates and this knowledge may define ways to improve performance of advanced formulated feeds.
DOI Link: 10.1017/s0007114520001051
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This article has been published in a revised form in British Journal of Nutrition [http://doi.org/10.1017/S0007114520001051]. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. © The Authors 2020.

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