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dc.contributor.authorWade, Nicholas Men_UK
dc.contributor.authorTrenkner, Lauren Hen_UK
dc.contributor.authorViegas, Ivanen_UK
dc.contributor.authorTavares, Ludgero Cen_UK
dc.contributor.authorPalma, Marianaen_UK
dc.contributor.authorSkiba-Cassy, Sandrineen_UK
dc.contributor.authorDias, Karineen_UK
dc.contributor.authorVachot, Christianeen_UK
dc.contributor.authorAraujo, Bruno Cen_UK
dc.contributor.authorBourne, Nicholasen_UK
dc.contributor.authorBlyth, Daviden_UK
dc.contributor.authorIrvin, Simonen_UK
dc.contributor.authorGlencross, Brett Den_UK
dc.description.abstractBarramundi (Lates calcarifer) are a highly valued aquaculture species, and, as obligate carnivores, they have a demonstrated preference for dietary protein over lipid or starch to fuel energetic growth demands. In order to investigate how carnivorous fish regulate nutritional cues, we examined the metabolic effects of feeding two isoenergetic diets that contained different proportions of digestible protein or starch energy. Fish fed a high proportion of dietary starch energy had a higher proportion of liver SFA, but showed no change in plasma glucose levels, and few changes in the expression of genes regulating key hepatic metabolic pathways. Decreased activation of the mammalian target of rapamycin growth signalling cascade was consistent with decreased growth performance values. The fractional synthetic rate (lipogenesis), measured by TAG 2H-enrichment using 2H NMR, was significantly higher in barramundi fed with the starch diet compared with the protein diet (0·6 (se 0·1) v. 0·4 (se 0·1) % per d, respectively). Hepatic TAG-bound glycerol synthetic rates were much higher than other closely related fish such as sea bass, but were not significantly different (starch, 2·8 (se 0·3) v. protein, 3·4 (se 0·3) % per d), highlighting the role of glycerol as a metabolic intermediary and high TAG-FA cycling in barramundi. Overall, dietary starch significantly increased hepatic TAG through increased lipogenesis. Compared with other fish, barramundi possess a unique mechanism to metabolise dietary carbohydrates and this knowledge may define ways to improve performance of advanced formulated feeds.en_UK
dc.publisherCambridge University Press (CUP)en_UK
dc.relationWade NM, Trenkner LH, Viegas I, Tavares LC, Palma M, Skiba-Cassy S, Dias K, Vachot C, Araujo BC, Bourne N, Blyth D, Irvin S & Glencross BD (2020) Dietary starch promotes hepatic lipogenesis in barramundi (Lates calcarifer). British Journal of Nutrition, 124 (4), pp. 363-373.
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This article has been published in a revised form in British Journal of Nutrition []. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. © The Authors 2020.en_UK
dc.subjectLipogenic fluxen_UK
dc.titleDietary starch promotes hepatic lipogenesis in barramundi (Lates calcarifer)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[dietary_starch_promotes_hepatic_lipogenesis_in_barramundi_lates_calcarifer.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.citation.jtitleBritish Journal of Nutritionen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderCommonwealth Scientific Industrial Research Organisationen_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.contributor.affiliationUniversity of Queenslanden_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.contributor.affiliationFrench National Institute for Agricultural Research (INRA)en_UK
dc.contributor.affiliationFrench National Institute for Agricultural Research (INRA)en_UK
dc.contributor.affiliationFrench National Institute for Agricultural Research (INRA)en_UK
dc.contributor.affiliationUniversity of Sao Pauloen_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
Appears in Collections:Aquaculture Journal Articles

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