|Appears in Collections:||Aquaculture Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence|
Monaghan, Sean J
|Citation:||Pang H, Li W, Zhang W, Zhou S, Hoare R, Monaghan SJ, Jian J & Lin X (2020) Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence. Journal of Proteomics, 211, Art. No.: 103543. https://doi.org/10.1016/j.jprot.2019.103543|
|Abstract:||It is well known that lysine acetylation (Kace) modification is a common post-translational modification (PTM) that plays an important role in multiple biological and pathological functions in bacteria. However, few studies have focused on lysine acetylation modification in aquatic pathogens to date. In this study, the acetylome profiling of fish pathogen, Vibrio alginolyticus was investigated by combining affinity enrichment with LC MS/MS. A total of 2883 acetylation modification sites on 1178 proteins in this pathogen were identified. The Kace modification of several selected proteins were further validated by Co-immunocoprecipitation combined with Western blotting. Bioinformatics analysis showed that seven conserved motifs can be enriched among Kace peptides, and many of them were significantly enriched in metabolic processes such as biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and biosynthesis of amino acids, which was similar to data previously published for V. parahaemolyticus. Moreover, we found at least 102 acetylation modified proteins predicted as virulence factors, which indicate the important role of PTM on bacterial virulence. In general, our results provide a promising starting point for further investigations of the biological role of lysine acetylation on bacterial virulence in V. alginolyticus.|
|Rights:||This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Pang H, Li W, Zhang W, Zhou S, Hoare R, Monaghan SJ, Jian J & Lin X (2020) Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence. Journal of Proteomics, 211, Art. No.: 103543. DOI: https://doi.org/10.1016/j.jprot.2019.103543 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/|
|20190923_VA_JP_last_version.pdf||Fulltext - Accepted Version||1.98 MB||Adobe PDF||View/Open|
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