Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30442
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dc.contributor.authorPang, Huanyingen_UK
dc.contributor.authorLi, Wanxinen_UK
dc.contributor.authorZhang, Wwijieen_UK
dc.contributor.authorZhou, Shihuien_UK
dc.contributor.authorHoare, Rowenaen_UK
dc.contributor.authorMonaghan, Sean Jen_UK
dc.contributor.authorJian, Jichangen_UK
dc.contributor.authorLin, Xiangminen_UK
dc.date.accessioned2019-11-08T01:00:19Z-
dc.date.available2019-11-08T01:00:19Z-
dc.date.issued2020-01-16en_UK
dc.identifier.other103543en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30442-
dc.description.abstractIt is well known that lysine acetylation (Kace) modification is a common post-translational modification (PTM) that plays an important role in multiple biological and pathological functions in bacteria. However, few studies have focused on lysine acetylation modification in aquatic pathogens to date. In this study, the acetylome profiling of fish pathogen, Vibrio alginolyticus was investigated by combining affinity enrichment with LC MS/MS. A total of 2883 acetylation modification sites on 1178 proteins in this pathogen were identified. The Kace modification of several selected proteins were further validated by Co-immunocoprecipitation combined with Western blotting. Bioinformatics analysis showed that seven conserved motifs can be enriched among Kace peptides, and many of them were significantly enriched in metabolic processes such as biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and biosynthesis of amino acids, which was similar to data previously published for V. parahaemolyticus. Moreover, we found at least 102 acetylation modified proteins predicted as virulence factors, which indicate the important role of PTM on bacterial virulence. In general, our results provide a promising starting point for further investigations of the biological role of lysine acetylation on bacterial virulence in V. alginolyticus.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationPang H, Li W, Zhang W, Zhou S, Hoare R, Monaghan SJ, Jian J & Lin X (2020) Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence. Journal of Proteomics, 211, Art. No.: 103543. https://doi.org/10.1016/j.jprot.2019.103543en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Pang H, Li W, Zhang W, Zhou S, Hoare R, Monaghan SJ, Jian J & Lin X (2020) Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence. Journal of Proteomics, 211, Art. No.: 103543. DOI: https://doi.org/10.1016/j.jprot.2019.103543 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectVibrio alginolyticusen_UK
dc.subjectAcetylomeen_UK
dc.subjectBacterial virulenceen_UK
dc.subjectProteomicsen_UK
dc.titleAcetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulenceen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-10-26en_UK
dc.rights.embargoreason[20190923_VA_JP_last_version.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.jprot.2019.103543en_UK
dc.identifier.pmid31669173en_UK
dc.citation.jtitleJournal of Proteomicsen_UK
dc.citation.issn1876-7737en_UK
dc.citation.issn1874-3919en_UK
dc.citation.volume211en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emails.j.monaghan@stir.ac.uken_UK
dc.citation.date25/10/2019en_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationGuangdong Ocean Universityen_UK
dc.contributor.affiliationFujian Agriculture and Forestry Universityen_UK
dc.identifier.scopusid2-s2.0-85074063736en_UK
dc.identifier.wtid1477504en_UK
dc.contributor.orcid0000-0002-9298-4275en_UK
dc.contributor.orcid0000-0002-7692-7756en_UK
dc.date.accepted2019-10-03en_UK
dc.description.refREF Compliant by Deposit in Stirling's Repositoryen_UK
dc.date.filedepositdate2019-11-07en_UK
Appears in Collections:Aquaculture Journal Articles

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