Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30099
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals
Author(s): Harper, Lynsey R
Lawson Handley, Lori
Carpenter, Angus I
Ghazali, Muhammad
Di Muri, Cristina
Macgregor, Callum J
Logan, Thomas W
Law, Alan
Breithaupt, Thomas
Read, Daniel S
McDevitt, Allan D
Hänfling, Bernd
Contact Email: alan.law1@stir.ac.uk
Keywords: Camera traps
Field signs
Lentic
Monitoring
Semi-aquatic mammals
Terrestrial mammals
Issue Date: Oct-2019
Citation: Harper LR, Lawson Handley L, Carpenter AI, Ghazali M, Di Muri C, Macgregor CJ, Logan TW, Law A, Breithaupt T, Read DS, McDevitt AD & Hänfling B (2019) Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals. Biological Conservation, 238, Art. No.: 108225. https://doi.org/10.1016/j.biocon.2019.108225
Abstract: Environmental DNA (eDNA) metabarcoding can identify terrestrial taxa utilising aquatic habitats alongside aquatic communities, but terrestrial species' eDNA dynamics are understudied. We evaluated eDNA metabarcoding for monitoring semi-aquatic and terrestrial mammals, specifically nine species of conservation or management concern, and examined spatiotemporal variation in mammal eDNA signals. We hypothesised eDNA signals would be stronger for semi-aquatic than terrestrial mammals, and at sites where individuals exhibited behaviours. In captivity, we sampled waterbodies at points where behaviours were observed (‘directed’ sampling) and at equidistant intervals along the shoreline (‘stratified’ sampling). We surveyed natural ponds (N = 6) where focal species were present using stratified water sampling, camera traps, and field signs. eDNA samples were metabarcoded using vertebrate-specific primers. All focal species were detected in captivity. eDNA signal strength did not differ between directed and stratified samples across or within species, between semi-aquatic or terrestrial species, or according to behaviours. eDNA was evenly distributed in artificial waterbodies, but unevenly distributed in natural ponds. Survey methods deployed at natural ponds shared three species detections. Metabarcoding missed badger and red fox recorded by cameras and field signs, but detected small mammals these tools overlooked, e.g. water vole. Terrestrial mammal eDNA signals were weaker and detected less frequently than semi-aquatic mammal eDNA signals. eDNA metabarcoding could enhance mammal monitoring through large-scale, multi-species distribution assessment for priority and difficult to survey species, and provide early indication of range expansions or contractions. However, eDNA surveys need high spatiotemporal resolution and metabarcoding biases require further investigation before routine implementation.
DOI Link: 10.1016/j.biocon.2019.108225
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Harper LR, Lawson Handley L, Carpenter AI, Ghazali M, Di Muri C, Macgregor CJ, Logan TW, Law A, Breithaupt T, Read DS, McDevitt AD & Hänfling B (2019) Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals. Biological Conservation, 238, Art. No.: 108225. DOI: https://doi.org/10.1016/j.biocon.2019.108225 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

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