Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29335
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity
Author(s): Ran, Zhaoshou
Xu, Jilin
Liao, Kai
Monroig, Óscar
Navarro, Juan Carlos
Oboh, Angela
Jin, Min
Zhou, Qicun
Zhou, Chengxu
Tocher, Douglas R
Yan, Xiaojun
Contact Email: d.r.tocher@stir.ac.uk
Keywords: docosahexaenoic acid biosynthesis
fatty acyl desaturases
elongation of very long-chain fatty acids protein
Long-chain polyunsaturated fatty acids
Sinonovacula constricta
Issue Date: Aug-2019
Citation: Ran Z, Xu J, Liao K, Monroig Ó, Navarro JC, Oboh A, Jin M, Zhou Q, Zhou C, Tocher DR & Yan X (2019) Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1864 (8), pp. 1083-1090. https://doi.org/10.1016/j.bbalip.2019.04.004
Abstract: As an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Here, we further successfully characterize elongases of very long-chain fatty acids (Elovl) in this important bivalve species, including one Elovl2/5, two Elovl4 isoforms (a and b) and a novel Elovl (c) with Elovl4 activity. In addition, we also determined the desaturation activity of S. constricta Δ6 Fad toward 24:5n-3 to give 24:6n-3, a key intermediate in docosahexaenoic acid (DHA) biosynthesis. Therefore, S. constricta is the first marine mollusc reported to possess all Fad and Elovl activities required for LC-PUFA biosynthesis via the ‘Sprecher pathway’. This finding greatly increases our understanding of LC-PUFA biosynthesis in marine molluscs. Phylogenetic analysis by interrogating six marine molluscan genomes, and previously functionally characterized Elovl and Fad from marine molluscs, suggested that DHA biosynthetic ability was limited to a few species, due to the general lack of Δ4 or Δ6 Fad in most molluscs.
DOI Link: 10.1016/j.bbalip.2019.04.004
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ran Z, Xu J, Liao K, Monroig Ó, Navarro JC, Oboh A, Jin M, Zhou Q, Zhou C, Tocher DR & Yan X (2019) Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1864 (8), pp. 1083-1090. DOI: https://doi.org/10.1016/j.bbalip.2019.04.004 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Files in This Item:
File Description SizeFormat 
Ran et al (Razor clam) REPOSITORIO.pdfFulltext - Accepted Version1.55 MBAdobe PDFView/Open



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.