Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29335
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dc.contributor.authorRan, Zhaoshouen_UK
dc.contributor.authorXu, Jilinen_UK
dc.contributor.authorLiao, Kaien_UK
dc.contributor.authorMonroig, Óscaren_UK
dc.contributor.authorNavarro, Juan Carlosen_UK
dc.contributor.authorOboh, Angelaen_UK
dc.contributor.authorJin, Minen_UK
dc.contributor.authorZhou, Qicunen_UK
dc.contributor.authorZhou, Chengxuen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorYan, Xiaojunen_UK
dc.date.accessioned2019-04-17T00:01:49Z-
dc.date.available2019-04-17T00:01:49Z-
dc.date.issued2019-08en_UK
dc.identifier.urihttp://hdl.handle.net/1893/29335-
dc.description.abstractAs an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Here, we further successfully characterize elongases of very long-chain fatty acids (Elovl) in this important bivalve species, including one Elovl2/5, two Elovl4 isoforms (a and b) and a novel Elovl (c) with Elovl4 activity. In addition, we also determined the desaturation activity of S. constricta Δ6 Fad toward 24:5n-3 to give 24:6n-3, a key intermediate in docosahexaenoic acid (DHA) biosynthesis. Therefore, S. constricta is the first marine mollusc reported to possess all Fad and Elovl activities required for LC-PUFA biosynthesis via the ‘Sprecher pathway’. This finding greatly increases our understanding of LC-PUFA biosynthesis in marine molluscs. Phylogenetic analysis by interrogating six marine molluscan genomes, and previously functionally characterized Elovl and Fad from marine molluscs, suggested that DHA biosynthetic ability was limited to a few species, due to the general lack of Δ4 or Δ6 Fad in most molluscs.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationRan Z, Xu J, Liao K, Monroig Ó, Navarro JC, Oboh A, Jin M, Zhou Q, Zhou C, Tocher DR & Yan X (2019) Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1864 (8), pp. 1083-1090. https://doi.org/10.1016/j.bbalip.2019.04.004en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ran Z, Xu J, Liao K, Monroig Ó, Navarro JC, Oboh A, Jin M, Zhou Q, Zhou C, Tocher DR & Yan X (2019) Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1864 (8), pp. 1083-1090. DOI: https://doi.org/10.1016/j.bbalip.2019.04.004 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectdocosahexaenoic acid biosynthesisen_UK
dc.subjectfatty acyl desaturasesen_UK
dc.subjectelongation of very long-chain fatty acids proteinen_UK
dc.subjectLong-chain polyunsaturated fatty acidsen_UK
dc.subjectSinonovacula constrictaen_UK
dc.titleBiosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacityen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-04-17en_UK
dc.rights.embargoreason[Ran et al (Razor clam) REPOSITORIO.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.bbalip.2019.04.004en_UK
dc.identifier.pmid31002943en_UK
dc.citation.jtitleBiochimica et Biophysica Acta Molecular and Cell Biology of Lipidsen_UK
dc.citation.issn1388-1981en_UK
dc.citation.volume1864en_UK
dc.citation.issue8en_UK
dc.citation.spage1083en_UK
dc.citation.epage1090en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaild.r.tocher@stir.ac.uken_UK
dc.citation.date16/04/2019en_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNingbo Universityen_UK
dc.identifier.isiWOS:000471355700001en_UK
dc.identifier.scopusid2-s2.0-85064618378en_UK
dc.identifier.wtid1269302en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted2019-01-06en_UK
dcterms.dateAccepted2019-01-06en_UK
dc.date.filedepositdate2019-04-12en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorRan, Zhaoshou|en_UK
local.rioxx.authorXu, Jilin|en_UK
local.rioxx.authorLiao, Kai|en_UK
local.rioxx.authorMonroig, Óscar|en_UK
local.rioxx.authorNavarro, Juan Carlos|en_UK
local.rioxx.authorOboh, Angela|en_UK
local.rioxx.authorJin, Min|en_UK
local.rioxx.authorZhou, Qicun|en_UK
local.rioxx.authorZhou, Chengxu|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorYan, Xiaojun|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2020-04-17en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2020-04-16en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2020-04-17|en_UK
local.rioxx.filenameRan et al (Razor clam) REPOSITORIO.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1388-1981en_UK
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