Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29234
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Recombinase polymerase amplification assay for rapid detection of Monkeypox virus
Author(s): Davi, Saskia Dede
Kissenkötter, Jonas
Faye, Martin
Böhlken-Fascher, Susanne
Stahl-Hennig, Christiane
Faye, Oumar
Faye, Ousmane
Sall, Amadou A
Weidmann, Manfred
Ademowo, Olusegun George
Hufert, Frank T
Czerny, Claus-Peter
Abd El Wahed, Ahmed
Contact Email: m.w.weidmann@stir.ac.uk
Keywords: Recombinase polymerase amplification assay
Monkeypox Virus
mobile suitcase
point of need
rapid detection system
Issue Date: Sep-2019
Citation: Davi SD, Kissenkötter J, Faye M, Böhlken-Fascher S, Stahl-Hennig C, Faye O, Faye O, Sall AA, Weidmann M, Ademowo OG, Hufert FT, Czerny C & Abd El Wahed A (2019) Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Journal of Clinical Virology, 95 (1), pp. 41-45. https://doi.org/10.1016/j.diagmicrobio.2019.03.015
Abstract: In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed. MPXV, an Orthopoxvirus, is a zoonotic dsDNA virus, which is listed as a biothreat agent. RPA was operated at a single constant temperature of 42°C and produced results within 3 to 10 minutes. The MPXV-RPA-assay was highly sensitive with a limit of detection of 16 DNA molecules/μl. The clinical performance of the MPXV-RPA-assay was tested using 47 sera and whole blood samples from humans collected during the recent MPXV outbreak in Nigeria as well as 48 plasma samples from monkeys some of which were experimentally infected with MPXV. The specificity of the MPXV-RPA-assay was 100% (50/50), while the sensitivity was 95% (43/45). This new MPXV-RPA-assay is fast and can be easily utilised at low resource settings using a solar powered mobile suitcase laboratory.
DOI Link: 10.1016/j.diagmicrobio.2019.03.015
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Davi SD, Kissenkötter J, Faye M, Böhlken-Fascher S, Stahl-Hennig C, Faye O, Faye O, Sall AA, Weidmann M, Ademowo OG, Hufert FT, Czerny C & Abd El Wahed A (2019) Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Journal of Clinical Virology, 95 (1), pp. 41-45. DOI: https://doi.org/10.1016/j.diagmicrobio.2019.03.015 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http:// creativecommons.org/licenses/by-nc-nd/4.0/
Licence URL(s): http://creativecommons.org/licenses/by-nc-nd/4.0/

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