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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/29234
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dc.contributor.authorDavi, Saskia Dedeen_UK
dc.contributor.authorKissenkötter, Jonasen_UK
dc.contributor.authorFaye, Martinen_UK
dc.contributor.authorBöhlken-Fascher, Susanneen_UK
dc.contributor.authorStahl-Hennig, Christianeen_UK
dc.contributor.authorFaye, Oumaren_UK
dc.contributor.authorFaye, Ousmaneen_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorAdemowo, Olusegun Georgeen_UK
dc.contributor.authorHufert, Frank Ten_UK
dc.contributor.authorCzerny, Claus-Peteren_UK
dc.contributor.authorAbd El Wahed, Ahmeden_UK
dc.date.accessioned2019-04-06T00:00:17Z-
dc.date.available2019-04-06T00:00:17Z-
dc.date.issued2019-09en_UK
dc.identifier.urihttp://hdl.handle.net/1893/29234-
dc.description.abstractIn this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed. MPXV, an Orthopoxvirus, is a zoonotic dsDNA virus, which is listed as a biothreat agent. RPA was operated at a single constant temperature of 42°C and produced results within 3 to 10 minutes. The MPXV-RPA-assay was highly sensitive with a limit of detection of 16 DNA molecules/μl. The clinical performance of the MPXV-RPA-assay was tested using 47 sera and whole blood samples from humans collected during the recent MPXV outbreak in Nigeria as well as 48 plasma samples from monkeys some of which were experimentally infected with MPXV. The specificity of the MPXV-RPA-assay was 100% (50/50), while the sensitivity was 95% (43/45). This new MPXV-RPA-assay is fast and can be easily utilised at low resource settings using a solar powered mobile suitcase laboratory.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationDavi SD, Kissenkötter J, Faye M, Böhlken-Fascher S, Stahl-Hennig C, Faye O, Faye O, Sall AA, Weidmann M, Ademowo OG, Hufert FT, Czerny C & Abd El Wahed A (2019) Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Journal of Clinical Virology, 95 (1), pp. 41-45. https://doi.org/10.1016/j.diagmicrobio.2019.03.015en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Davi SD, Kissenkötter J, Faye M, Böhlken-Fascher S, Stahl-Hennig C, Faye O, Faye O, Sall AA, Weidmann M, Ademowo OG, Hufert FT, Czerny C & Abd El Wahed A (2019) Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Journal of Clinical Virology, 95 (1), pp. 41-45. DOI: https://doi.org/10.1016/j.diagmicrobio.2019.03.015 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http:// creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectRecombinase polymerase amplification assayen_UK
dc.subjectMonkeypox Virusen_UK
dc.subjectmobile suitcaseen_UK
dc.subjectpoint of needen_UK
dc.subjectrapid detection systemen_UK
dc.titleRecombinase polymerase amplification assay for rapid detection of Monkeypox virusen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-04-12en_UK
dc.rights.embargoreason[MPXV-Manuscript2 after revierwers corrections.pdf] Until this work is published there will be an embargo on the full text of this work. Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.diagmicrobio.2019.03.015en_UK
dc.identifier.pmid31126795en_UK
dc.citation.jtitleJournal of Clinical Virologyen_UK
dc.citation.issn1386-6532en_UK
dc.citation.volume95en_UK
dc.citation.issue1en_UK
dc.citation.spage41en_UK
dc.citation.epage45en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.citation.date11/04/2019en_UK
dc.contributor.affiliationBrandenburg Medical School Theodor-Fontaneen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationGerman Primate Centeren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Ibadan, Nigeriaen_UK
dc.contributor.affiliationBrandenburg Medical School Theodor-Fontaneen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.identifier.isiWOS:000483427000010en_UK
dc.identifier.scopusid2-s2.0-85065824423en_UK
dc.identifier.wtid1264473en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2019-04-04en_UK
dcterms.dateAccepted2019-04-04en_UK
dc.date.filedepositdate2019-04-05en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorDavi, Saskia Dede|en_UK
local.rioxx.authorKissenkötter, Jonas|en_UK
local.rioxx.authorFaye, Martin|en_UK
local.rioxx.authorBöhlken-Fascher, Susanne|en_UK
local.rioxx.authorStahl-Hennig, Christiane|en_UK
local.rioxx.authorFaye, Oumar|en_UK
local.rioxx.authorFaye, Ousmane|en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorAdemowo, Olusegun George|en_UK
local.rioxx.authorHufert, Frank T|en_UK
local.rioxx.authorCzerny, Claus-Peter|en_UK
local.rioxx.authorAbd El Wahed, Ahmed|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2020-04-12en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2020-04-11en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2020-04-12|en_UK
local.rioxx.filenameMPXV-Manuscript2 after revierwers corrections.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1386-6532en_UK
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