|Appears in Collections:
|Aquaculture Journal Articles
|Peer Review Status:
|Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)
|Jordal, Ann-Elise O
Torstensen, Bente E
Tocher, Douglas R
Lall, Santosh P
Douglas, Susan E
Fishes Feeding and feeds
|Jordal AO, Torstensen BE, Tsoi S, Tocher DR, Lall SP & Douglas SE (2005) Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.). Journal of Nutrition, 135 (10), pp. 2355-2361. http://jn.nutrition.org/content/135/10/2355.abstract
|Supplies of marine fish oils (FO ) are limited, and sustainable production in aquaculture dictates that alternatives, such as vegetable oils, must be found that do not compromise fish health and product quality. Nutrigenomics is anticipated to provide an increased understanding of how nutrition influences metabolic pathways and homeostatic control, and may be used to measure and validate subtle changes in organ-specific, metabolic gene expression signatures. We have compared two groups of Atlantic salmon fed diets containing 100% FO or 75% rapeseed oil (RO)/25% FO for 42 weeks. A small-scale cDNA microarray was constructed to screen for changes in expression of lipid metabolism genes in the liver resulting from this partial substitution of RO for FO. Fatty acid desaturase gene expression was significantly increased in fish fed 75% RO compared to fish fed the control diet; this was confirmed by quantitative Real Time PCR analysis (Q-PCR). In addition, several genes, among these mitochondrial proteins, peroxisome proliferator activated receptor-γ (PPARγ) as well as other transcription factors, co-activators and signal transducers, showed significant differential regulation. This partially validated microarray may be used for further gene expression profiling using other dietary comparisons.
|The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.
|Fulltext - Accepted Version
|Under Embargo until 3000-01-01 Request a copy
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/
If you believe that any material held in STORRE infringes copyright, please contact firstname.lastname@example.org providing details and we will remove the Work from public display in STORRE and investigate your claim.