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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Expanding the miRNA Repertoire in Atlantic Salmon; Discovery of IsomiRs and miRNAs Highly Expressed in Different Tissues and Developmental Stages
Author(s): Woldemariam, Nardos Tesfaye
Agafonov, Oleg
Hoyheim, Bjørn
Houston, Ross D
Taggart, John B
Andreassen, Rune
Keywords: Teleostei
tissue-enriched miRNAs
post-transcriptional gene regulation
Issue Date: 11-Jan-2019
Date Deposited: 30-Jan-2019
Citation: Woldemariam NT, Agafonov O, Hoyheim B, Houston RD, Taggart JB & Andreassen R (2019) Expanding the miRNA Repertoire in Atlantic Salmon; Discovery of IsomiRs and miRNAs Highly Expressed in Different Tissues and Developmental Stages. Cells, 8 (1), Art. No.: 42.
Abstract: MicroRNAs (miRNAs) are important post-transcriptional gene expression regulators. Here, 448 different miRNA genes, including 17 novel miRNAs, encoding for 589 mature Atlantic salmon miRNAs were identified after sequencing 111 samples (fry, pathogen challenged fry, various developmental and adult tissues). This increased the reference miRNAome with almost one hundred genes. Prior to isomiR characterization (mature miRNA variants), the proportion of erroneous sequence variants (ESVs) arising in the analysis pipeline was assessed. The ESVs were biased towards 5’ and 3’ end of reads in unexpectedly high proportions indicating that measurements of ESVs rather than Phred score should be used to avoid misinterpreting ESVs as isomiRs. Forty-three isomiRs were subsequently discovered. The biological effect of the isomiRs measured as increases in target diversity was small (
DOI Link: 10.3390/cells8010042
Rights: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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