Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27794
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Molecular epidemiological study on Infectious Pancreatic Necrosis Virus isolates from aquafarms in Scotland over three decades
Author(s): Ulrich, Kristina
Wehner, Stefanie
Bekaert, Michaël
Paola, Nicholas Di
Dilcher, Meik
Muir, Katherine Fiona
Taggart, John B
Matejusova, Iveta
Weidmann, Manfred
Keywords: Infectious Pancreatic Necrosis Virus
IPNV
Salmon
evolution
Codon adaptation
phylogeny
selection
vaccine
adaptation
Aquaculture
Issue Date: 1-Dec-2018
Citation: Ulrich K, Wehner S, Bekaert M, Paola ND, Dilcher M, Muir KF, Taggart JB, Matejusova I & Weidmann M (2018) Molecular epidemiological study on Infectious Pancreatic Necrosis Virus isolates from aquafarms in Scotland over three decades. Journal of General Virology, 99 (12), pp. 1567-1581. https://doi.org/10.1099/jgv.0.001155.
Abstract: In order to obtain an insight into genomic changes and associated evolution and adaptation of Infectious Pancreatic Necrosis Virus (IPNV) the complete coding genomes of 57 IPNV isolates collected from Scottish aquafarms from 1982-2014 were sequenced and analysed. Phylogenetic analysis of the sequenced IPNV strains showed separate clustering of genogroups I, II, III and V. IPNV isolates with genetic reassortment of segment A/B of genogroup III/II were determined. About 59% of the IPNV isolates belonged to the persistent type and 32% to the low virulent type and only one highly pathogenic strain0 (1.79%) was identified. Codon adaptation index calculations indicated that the IPNV major capsid protein VP2 has adapted to its salmonid host. Underrepresentation of CpG dinucleotides in the IPNV genome to minimise detection by the innate immunity receptors, and observed positive selection in the virulence determination sites of VP2 embedded in the variable region of the main antigenic region, suggest an immune escape mechanism driving virulence evolution. The prevalence of mostly persistent genotypes together with the assumption of adaptation and immune escape indicates that IPNV is evolving with the host.
DOI Link: 10.1099/jgv.0.001155
Rights: Publisher allows this work to be made available in this repository. Published in Journal of General Virology by Microbiology Society with the following policy: We permit all forms of non-commercial reuse of pre-prints and post-prints, including non-commercial text and data mining. This includes use for the purpose of research, teaching or other related activity, but not use for the purposes of monetary reward by means of sale, resale, loan, transfer, hire or other form of exploitation. The published version is available at: https://doi.org/10.1099/jgv.0.001155

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