Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27612
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis)
Author(s): Carmona-Antoñanzas, Greta
Helgesen, Kari O
Humble, Joseph L
Tschesche, Claudia
Bakke, Marit J
Gamble, Louise
Bekaert, Michaël
Bassett, David I
Horsberg, Tor E
Bron, James E
Sturm, Armin
Contact Email: armin.sturm@stir.ac.uk
Keywords: Agronomy and Crop Science
Insect Science
General Medicine
Issue Date: 1-Feb-2019
Citation: Carmona-Antoñanzas G, Helgesen KO, Humble JL, Tschesche C, Bakke MJ, Gamble L, Bekaert M, Bassett DI, Horsberg TE, Bron JE & Sturm A (2019) Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75 (2), pp. 527-536. https://doi.org/10.1002/ps.5151
Abstract: Background Parasitic salmon lice (Lepeophtheirus salmonis) cause high economic losses in Atlantic salmon farming. Pyrethroids, which block arthropod voltage‐gated sodium channels (Nav1), are used for salmon delousing. However, pyrethroid resistance is common in L. salmonis. The present study characterised Nav1 homologues in L. salmonis in order to identify channel mutations associated to resistance, called kdr (knockdown) mutations. Results Genome scans identified three L. salmonis Nav1 homologues, LsNav1.1, LsNav1.2 and LsNav1.3. Arthropod kdr mutations map to specific Nav1 regions within domains DI‐III, namely segments S5 and S6 and the linker helix connecting S4 and S5. The above channel regions were amplified by RT‐PCR and sequenced in deltamethrin‐susceptible and deltamethrin‐resistant L. salmonis. While LsNav1.1 and LsNav1.2 lacked nucleotide polymorphisms showing association to resistance, LsNav1.3 showed a non‐synonymous mutation in S5 of DII occurring in deltamethrin‐resistant parasites. The mutation is homologous to a previously described kdr mutation (I936V, numbering according to Musca domestica Vssc1) and was present in two pyrethroid‐resistant L. salmonis strains (allele frequencies of 0.800 and 0.357), but absent in two pyrethroid‐susceptible strains. Conclusions The present study indicates that a kdr‐mutation in LsNaV 1.3 may contribute to deltamethrin resistance in L. salmonis.
DOI Link: 10.1002/ps.5151
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Carmona‐Antoñanzas, G. , Helgesen, K. O., Humble, J. L., Tschesche, C. , Bakke, M. J., Gamble, L. , Bekaert, M. , Bassett, D. I., Horsberg, T. E., Bron, J. E. and Sturm, A. (2019), Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75: 527-536, which has been published in final form at https://doi.org/10.1002/ps.5151. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving.

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