Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27612
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dc.contributor.authorCarmona-Antoñanzas, Gretaen_UK
dc.contributor.authorHelgesen, Kari Oen_UK
dc.contributor.authorHumble, Joseph Len_UK
dc.contributor.authorTschesche, Claudiaen_UK
dc.contributor.authorBakke, Marit Jen_UK
dc.contributor.authorGamble, Louiseen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorBassett, David Ien_UK
dc.contributor.authorHorsberg, Tor Een_UK
dc.contributor.authorBron, James Een_UK
dc.contributor.authorSturm, Arminen_UK
dc.date.accessioned2018-08-14T00:02:36Z-
dc.date.available2018-08-14T00:02:36Z-
dc.date.issued2019-02-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/27612-
dc.description.abstractBackground Parasitic salmon lice (Lepeophtheirus salmonis) cause high economic losses in Atlantic salmon farming. Pyrethroids, which block arthropod voltage‐gated sodium channels (Nav1), are used for salmon delousing. However, pyrethroid resistance is common in L. salmonis. The present study characterised Nav1 homologues in L. salmonis in order to identify channel mutations associated to resistance, called kdr (knockdown) mutations. Results Genome scans identified three L. salmonis Nav1 homologues, LsNav1.1, LsNav1.2 and LsNav1.3. Arthropod kdr mutations map to specific Nav1 regions within domains DI‐III, namely segments S5 and S6 and the linker helix connecting S4 and S5. The above channel regions were amplified by RT‐PCR and sequenced in deltamethrin‐susceptible and deltamethrin‐resistant L. salmonis. While LsNav1.1 and LsNav1.2 lacked nucleotide polymorphisms showing association to resistance, LsNav1.3 showed a non‐synonymous mutation in S5 of DII occurring in deltamethrin‐resistant parasites. The mutation is homologous to a previously described kdr mutation (I936V, numbering according to Musca domestica Vssc1) and was present in two pyrethroid‐resistant L. salmonis strains (allele frequencies of 0.800 and 0.357), but absent in two pyrethroid‐susceptible strains. Conclusions The present study indicates that a kdr‐mutation in LsNaV 1.3 may contribute to deltamethrin resistance in L. salmonis.en_UK
dc.language.isoenen_UK
dc.publisherWileyen_UK
dc.relationCarmona-Antoñanzas G, Helgesen KO, Humble JL, Tschesche C, Bakke MJ, Gamble L, Bekaert M, Bassett DI, Horsberg TE, Bron JE & Sturm A (2019) Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75 (2), pp. 527-536. https://doi.org/10.1002/ps.5151en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Carmona‐Antoñanzas, G. , Helgesen, K. O., Humble, J. L., Tschesche, C. , Bakke, M. J., Gamble, L. , Bekaert, M. , Bassett, D. I., Horsberg, T. E., Bron, J. E. and Sturm, A. (2019), Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75: 527-536, which has been published in final form at https://doi.org/10.1002/ps.5151. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving.en_UK
dc.subjectAgronomy and Crop Scienceen_UK
dc.subjectInsect Scienceen_UK
dc.subjectGeneral Medicineen_UK
dc.titleMutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2019-08-01en_UK
dc.rights.embargoreason[Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1002/ps.5151en_UK
dc.identifier.pmid30062864en_UK
dc.citation.jtitlePest Management Scienceen_UK
dc.citation.issn1526-4998en_UK
dc.citation.issn1526-498Xen_UK
dc.citation.volume75en_UK
dc.citation.issue2en_UK
dc.citation.spage527en_UK
dc.citation.epage536en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen_UK
dc.author.emailarmin.sturm@stir.ac.uken_UK
dc.citation.date31/07/2018en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNorwegian Veterinary Instituteen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNorwegian University of Life Sciencesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationNorwegian University of Life Sciencesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000455406600001en_UK
dc.identifier.scopusid2-s2.0-85053841945en_UK
dc.identifier.wtid970527en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.contributor.orcid0000-0002-7529-0829en_UK
dc.contributor.orcid0000-0003-3544-0519en_UK
dc.contributor.orcid0000-0003-2632-1999en_UK
dc.date.accepted2018-07-19en_UK
dcterms.dateAccepted2018-07-19en_UK
dc.date.filedepositdate2018-08-13en_UK
dc.relation.funderprojectIdentifying molecular determinants of drug susceptibility in salmon lice (Lepeophtheirus salmonis)en_UK
dc.relation.funderrefBB/L022923/1en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorCarmona-Antoñanzas, Greta|en_UK
local.rioxx.authorHelgesen, Kari O|en_UK
local.rioxx.authorHumble, Joseph L|en_UK
local.rioxx.authorTschesche, Claudia|en_UK
local.rioxx.authorBakke, Marit J|en_UK
local.rioxx.authorGamble, Louise|en_UK
local.rioxx.authorBekaert, Michaël|0000-0002-1206-7654en_UK
local.rioxx.authorBassett, David I|0000-0002-7529-0829en_UK
local.rioxx.authorHorsberg, Tor E|en_UK
local.rioxx.authorBron, James E|0000-0003-3544-0519en_UK
local.rioxx.authorSturm, Armin|0000-0003-2632-1999en_UK
local.rioxx.projectBB/L022923/1|Biotechnology and Biological Sciences Research Council|http://dx.doi.org/10.13039/501100000268en_UK
local.rioxx.freetoreaddate2019-08-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2019-07-31en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2019-08-01|en_UK
local.rioxx.filenameCarmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1526-498Xen_UK
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