Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/27545
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines
Author(s): Attaya, Ahmed
Wang, Tiehui
Zou, Jun
Herath, Tharangani
Adams, Alexandra
Secombes, Christopher J
Yoon, Sohye
Contact Email: alexandra.adams@stir.ac.uk
Keywords: GALT leucocytes
Isolation
Salmonids
PAMPs
Recombinant cytokines
Gene expression
Issue Date: 1-Sep-2018
Citation: Attaya A, Wang T, Zou J, Herath T, Adams A, Secombes CJ & Yoon S (2018) Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines. Fish & shellfish immunology, 80, pp. 426-436. https://doi.org/10.1016/j.fsi.2018.06.022
Abstract: Increased knowledge of the immune response of the intestine, a physiologically critical organ involved in absorption, secretion and homeostasis in a non-sterile environment, is needed to better understand the mechanisms involved in the induction of long-lasting immunity and, subsequently, the development of efficacious gastrointestinal immunization approaches. To this end, analysis of isolated gut cells will give an insight into the cell types present and their immune capability. Hence, in this study we first optimised a method for salmonid gut leucocyte isolation and characterised the cells on the basis of their expression of a range of selected cell markers associated with T & B cells and dendritic cells. The GALT leucocytes were then stimulated with a variety of PAMPs, recombinant cytokines and PHA, as a means to help characterise the diversity of the immune repertoire present in such cells. The stimulants tested were designed to examine the nature of the antibacterial, antiviral and T cell type responses in the cells (at the transcript level) using a panel of genes relevant to innate and adaptive immunity. The results showed distinct responses to the stimulants, with a clear delineation seen between the stimulant used (eg viral or bacterial PAMP) and the pathway elicited. The changes in the expression patterns of the immune genes in these cells indicates that the salmonid intestine contains a good repertoire of competent immune cells able to respond to different pathogen types. Such information may aid the development of efficient priming by oral vaccination in salmonids.
DOI Link: 10.1016/j.fsi.2018.06.022
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Attaya A, Wang T, Zou J, Herath T, Adams A, Secombes CJ & Yoon S (2018) Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines, Fish & Shellfish Immunology, 80, pp. 426-436. DOI: 10.1016/j.fsi.2018.06.022 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Licence URL(s): http://creativecommons.org/licenses/by-nc-nd/4.0/

Files in This Item:
File Description SizeFormat 
FSIM-D-18-00347R1.pdfFulltext - Accepted Version1.92 MBAdobe PDFView/Open



This item is protected by original copyright



A file in this item is licensed under a Creative Commons License Creative Commons

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.