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dc.contributor.authorAttaya, Ahmeden_UK
dc.contributor.authorWang, Tiehuien_UK
dc.contributor.authorZou, Junen_UK
dc.contributor.authorHerath, Tharanganien_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorSecombes, Christopher Jen_UK
dc.contributor.authorYoon, Sohyeen_UK
dc.description.abstractIncreased knowledge of the immune response of the intestine, a physiologically critical organ involved in absorption, secretion and homeostasis in a non-sterile environment, is needed to better understand the mechanisms involved in the induction of long-lasting immunity and, subsequently, the development of efficacious gastrointestinal immunization approaches. To this end, analysis of isolated gut cells will give an insight into the cell types present and their immune capability. Hence, in this study we first optimised a method for salmonid gut leucocyte isolation and characterised the cells on the basis of their expression of a range of selected cell markers associated with T & B cells and dendritic cells. The GALT leucocytes were then stimulated with a variety of PAMPs, recombinant cytokines and PHA, as a means to help characterise the diversity of the immune repertoire present in such cells. The stimulants tested were designed to examine the nature of the antibacterial, antiviral and T cell type responses in the cells (at the transcript level) using a panel of genes relevant to innate and adaptive immunity. The results showed distinct responses to the stimulants, with a clear delineation seen between the stimulant used (eg viral or bacterial PAMP) and the pathway elicited. The changes in the expression patterns of the immune genes in these cells indicates that the salmonid intestine contains a good repertoire of competent immune cells able to respond to different pathogen types. Such information may aid the development of efficient priming by oral vaccination in salmonids.en_UK
dc.relationAttaya A, Wang T, Zou J, Herath T, Adams A, Secombes CJ & Yoon S (2018) Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines. Fish & shellfish immunology, 80, pp. 426-436.
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Attaya A, Wang T, Zou J, Herath T, Adams A, Secombes CJ & Yoon S (2018) Gene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokines, Fish & Shellfish Immunology, 80, pp. 426-436. DOI: 10.1016/j.fsi.2018.06.022 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.subjectGALT leucocytesen_UK
dc.subjectRecombinant cytokinesen_UK
dc.subjectGene expressionen_UK
dc.titleGene expression analysis of isolated salmonid GALT leucocytes in response to PAMPs and recombinant cytokinesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[FSIM-D-18-00347R1.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.citation.jtitleFish and Shellfish Immunologyen_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.contributor.affiliationUniversity of Aberdeenen_UK
dc.relation.funderprojectDevelopment of novel oral vaccination strategies for Atlantic salmonen_UK
Appears in Collections:Aquaculture Journal Articles

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