Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26515
Appears in Collections:Psychology Journal Articles
Peer Review Status: Refereed
Title: Measuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay technique
Author(s): Ash, Hayley
Smith, Tessa E
Knight, Simon
Buchanan-Smith, Hannah M
Contact Email: h.m.buchanan-smith@stir.ac.uk
Keywords: Common marmoset
HPA axis
Salivary cortisol
Elisa
Swabs
Validation
Issue Date: 1-Mar-2018
Citation: Ash H, Smith TE, Knight S & Buchanan-Smith HM (2018) Measuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay technique, Physiology and Behavior, 185, pp. 14-22.
Abstract: Cortisol levels are often used as a physiological measure of the stress response in captive primates, with non-invasive measures of this being an important step in welfare assessment. We report a method of collecting saliva samples voluntarily from unrestrained captive common marmosets ( Callithrix jacchus), and validate an enzyme-linked immunosorbent assay (ELISA) technique previously unused in this species. Saliva samples were collected from marmosets housed in pairs in a UK laboratory. The assay showed parallelism, precision, accuracy and sensitivity, meeting the criteria typically used to investigate the effectiveness of new analytical techniques. Use of Salimetrics® Oral Swabs considerably increased the amount of cortisol recovered in comparison with previous studies using cotton buds. However, while use of banana on the swabs can encourage chewing, it may influence results. Although increases in cortisol levels have traditionally been interpreted as an indicator of stress in primates, there are many factors that affect the hypothalamic-pituitary-adrenal axis, with some studies showing decreases in cortisol levels post-stressor. Following a likely stressful event (capture for weighing), we also found cortisol levels significantly decreased, possibly due to social buffering or ‘blunting’ of the HPA axis. Order of weighing also had an effect. The method therefore provided an effective non-invasive means of assessing acute changes in cortisol level that may be more useful than previous methods, improving our ability to study physiological aspects of welfare in primates. We discuss methodological considerations, as well as implications of using cortisol as a measure of stress.
DOI Link: http://dx.doi.org/10.1016/j.physbeh.2017.12.018
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ash H, Smith TE, Knight S & Buchanan-Smith HM (2018) Measuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay technique, Physiology and Behavior, 185, pp. 14-22. DOI: 10.1016/j.physbeh.2017.12.018 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

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