Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/26515
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dc.contributor.authorAsh, Hayleyen_UK
dc.contributor.authorSmith, Tessa Een_UK
dc.contributor.authorKnight, Simonen_UK
dc.contributor.authorBuchanan-Smith, Hannah Men_UK
dc.date.accessioned2018-02-18T08:07:08Z-
dc.date.available2018-02-18T08:07:08Z-
dc.date.issued2018-03-01en_UK
dc.identifier.urihttp://hdl.handle.net/1893/26515-
dc.description.abstractCortisol levels are often used as a physiological measure of the stress response in captive primates, with non-invasive measures of this being an important step in welfare assessment. We report a method of collecting saliva samples voluntarily from unrestrained captive common marmosets (Callithrix jacchus), and validate an enzyme-linked immunosorbent assay (ELISA) technique previously unused in this species. Saliva samples were collected from marmosets housed in pairs in a UK laboratory. The assay showed parallelism, precision, accuracy and sensitivity, meeting the criteria typically used to investigate the effectiveness of new analytical techniques. Use of Salimetrics® Oral Swabs considerably increased the amount of cortisol recovered in comparison with previous studies using cotton buds. However, while use of banana on the swabs can encourage chewing, it may influence results. Although increases in cortisol levels have traditionally been interpreted as an indicator of stress in primates, there are many factors that affect the hypothalamic-pituitary-adrenal axis, with some studies showing decreases in cortisol levels post-stressor. Following a likely stressful event (capture for weighing), we also found cortisol levels significantly decreased, possibly due to social buffering or ‘blunting’ of the HPA axis. Order of weighing also had an effect. The method therefore provided an effective non-invasive means of assessing acute changes in cortisol level that may be more useful than previous methods, improving our ability to study physiological aspects of welfare in primates. We discuss methodological considerations, as well as implications of using cortisol as a measure of stress.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationAsh H, Smith TE, Knight S & Buchanan-Smith HM (2018) Measuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay technique. Physiology and Behavior, 185, pp. 14-22. https://doi.org/10.1016/j.physbeh.2017.12.018en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Ash H, Smith TE, Knight S & Buchanan-Smith HM (2018) Measuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay technique, Physiology and Behavior, 185, pp. 14-22. DOI: 10.1016/j.physbeh.2017.12.018 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectCommon marmoseten_UK
dc.subjectHPA axisen_UK
dc.subjectSalivary cortisolen_UK
dc.subjectElisaen_UK
dc.subjectSwabsen_UK
dc.subjectValidationen_UK
dc.titleMeasuring physiological stress in the common marmoset (Callithrix jacchus): Validation of a salivary cortisol collection and assay techniqueen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2018-12-16en_UK
dc.rights.embargoreason[Cortisol manuscript for Physiology and Behavior revision clean.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.physbeh.2017.12.018en_UK
dc.identifier.pmid29248632en_UK
dc.citation.jtitlePhysiology and Behavioren_UK
dc.citation.issn0031-9384en_UK
dc.citation.volume185en_UK
dc.citation.spage14en_UK
dc.citation.epage22en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emailh.m.buchanan-smith@stir.ac.uken_UK
dc.citation.date15/12/2017en_UK
dc.contributor.affiliationPsychologyen_UK
dc.contributor.affiliationUniversity of Chesteren_UK
dc.contributor.affiliationPorton Downen_UK
dc.contributor.affiliationPsychologyen_UK
dc.identifier.isiWOS:000423889200002en_UK
dc.identifier.scopusid2-s2.0-85038817578en_UK
dc.identifier.wtid507688en_UK
dc.contributor.orcid0000-0002-2516-7734en_UK
dc.date.accepted2017-12-14en_UK
dc.date.filedepositdate2018-01-15en_UK
Appears in Collections:Psychology Journal Articles

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