Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?
Author(s): Verner-Jeffreys, David W
Brazier, Thomas
Perez, Ramon Y
Ryder, David
Card, Roderick M
Welch, Timothy J
Hoare, Rowena
Ngo, Thao P H
McLaren, Nikki
Ellis, Richard
Bartie, Kerry
Feist, Stephen W
Rowe, William M P
Adams, Alexandra
Thompson, Kim D
Contact Email:
Keywords: Flavobacteriaceae
fish pathogen
antimicrobial resistance
horizontal gene transfer
Issue Date: Apr-2017
Citation: Verner-Jeffreys DW, Brazier T, Perez RY, Ryder D, Card RM, Welch TJ, Hoare R, Ngo TPH, McLaren N, Ellis R, Bartie K, Feist SW, Rowe WMP, Adams A & Thompson KD (2017) Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?, FEMS Microbiology Ecology, 93 (4), Art. No.: fix015.
Abstract: Bacteria from the family Flavobacteriaceae often show low susceptibility to antibiotics. With the exception of two Chryseobacterium spp. isolates that were positive for the florfenicol resistance gene floR, no clinical resistance genes were identified by microarray in 36 Flavobacteriaceae isolates from salmonid fish that could grow in ≥ 4 mg/L florfenicol. Whole genome sequence analysis of the floR positive isolates revealed the presence of a region that contained the antimicrobial resistance genes floR, a tet(X) tetracycline resistance gene, a streptothricin resistance gene and a chloramphenicol acetyltransferase gene. In silico analysis of 377 published genomes for Flavobacteriaceae isolates from a range of sources confirmed that well-characterised resistance gene cassettes were not widely distributed in bacteria from this group. Efflux pump-mediated decreased susceptibility to a range of antimicrobials was confirmed in both floR positive isolates using an efflux pump inhibitor (phenylalanine-arginine β-naphthylamide) assay. The floR isolates possessed putative virulence factors, including production of siderophores and haemolysins, and were mildly pathogenic in rainbow trout. Results support the suggestion that, despite the detection of floR, susceptibility to antimicrobials in Flavobacteriaceae is mostly mediated via intrinsic mechanisms rather than the horizontally acquired resistance genes more normally associated with Gram-negative bacterial pathogens such as Enterobacteriaceae.
DOI Link:
Rights: Use in this Repository permitted under the Open Government Licence:

Files in This Item:
File Description SizeFormat 
Accepted MS .pdf1.25 MBAdobe PDFView/Open
fix015.pdf1.5 MBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.