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STORRE: Stirling Online Research Repository
http://hdl.handle.net/1893/25189| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA) |
| Author(s): | Bergmann, Sven M Wang, Qing Zeng, Weiwei Li, Yingying Wang, Yingying Matras, Marek Reichert, Michal Fichtner, Dieter Lenk, Matthias Morin, Thierry Olesen, Niels Jorgen Skall, Helle F Lee, Pei-Yu Zheng, Shuchen Monaghan, Sean |
| Contact Email: | s.j.monaghan@stir.ac.uk |
| Keywords: | carp and koi sera enzyme-linked immunosorbent assay koi herpesvirus koi herpesvirus antibodies |
| Issue Date: | Nov-2017 |
| Date Deposited: | 22-Mar-2017 |
| Citation: | Bergmann SM, Wang Q, Zeng W, Li Y, Wang Y, Matras M, Reichert M, Fichtner D, Lenk M, Morin T, Olesen NJ, Skall HF, Lee P, Zheng S & Monaghan S (2017) Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA). Journal of Fish Diseases, 40 (11), pp. 1511-1527. https://doi.org/10.1111/jfd.12621 |
| Abstract: | Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs. |
| DOI Link: | 10.1111/jfd.12621 |
| Rights: | The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. |
| Notes: | Additional co-authors: S Reiche, W Fuchs, M Kotler, K Way, G Bräuer, K Böttcher, A Kappe, and J Kielpinska |
| Licence URL(s): | http://www.rioxx.net/licenses/under-embargo-all-rights-reserved |
| File | Description | Size | Format | |
|---|---|---|---|---|
| Bergmann_et_al-2017-Journal_of_Fish_Diseases.pdf | Fulltext - Published Version | 479.71 kB | Adobe PDF | Under Embargo until 2999-12-08 Request a copy |
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