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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/25189
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dc.contributor.authorBergmann, Sven Men_UK
dc.contributor.authorWang, Qingen_UK
dc.contributor.authorZeng, Weiweien_UK
dc.contributor.authorLi, Yingyingen_UK
dc.contributor.authorWang, Yingyingen_UK
dc.contributor.authorMatras, Mareken_UK
dc.contributor.authorReichert, Michalen_UK
dc.contributor.authorFichtner, Dieteren_UK
dc.contributor.authorLenk, Matthiasen_UK
dc.contributor.authorMorin, Thierryen_UK
dc.contributor.authorOlesen, Niels Jorgenen_UK
dc.contributor.authorSkall, Helle Fen_UK
dc.contributor.authorLee, Pei-Yuen_UK
dc.contributor.authorZheng, Shuchenen_UK
dc.contributor.authorMonaghan, Seanen_UK
dc.date.accessioned2017-11-11T23:11:40Z-
dc.date.available2017-11-11T23:11:40Zen_UK
dc.date.issued2017-11en_UK
dc.identifier.urihttp://hdl.handle.net/1893/25189-
dc.description.abstractKoi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.en_UK
dc.language.isoenen_UK
dc.publisherWiley-Blackwellen_UK
dc.relationBergmann SM, Wang Q, Zeng W, Li Y, Wang Y, Matras M, Reichert M, Fichtner D, Lenk M, Morin T, Olesen NJ, Skall HF, Lee P, Zheng S & Monaghan S (2017) Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA). Journal of Fish Diseases, 40 (11), pp. 1511-1527. https://doi.org/10.1111/jfd.12621en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectcarp and koi seraen_UK
dc.subjectenzyme-linked immunosorbent assayen_UK
dc.subjectkoi herpesvirusen_UK
dc.subjectkoi herpesvirus antibodiesen_UK
dc.titleValidation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-08en_UK
dc.rights.embargoreason[Bergmann_et_al-2017-Journal_of_Fish_Diseases.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1111/jfd.12621en_UK
dc.identifier.pmid28470973en_UK
dc.citation.jtitleJournal of Fish Diseasesen_UK
dc.citation.issn1365-2761en_UK
dc.citation.issn0140-7775en_UK
dc.citation.volume40en_UK
dc.citation.issue11en_UK
dc.citation.spage1511en_UK
dc.citation.epage1527en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emails.j.monaghan@stir.ac.uken_UK
dc.citation.date07/05/2017en_UK
dc.description.notesAdditional co-authors: S Reiche, W Fuchs, M Kotler, K Way, G Bräuer, K Böttcher, A Kappe, and J Kielpinskaen_UK
dc.contributor.affiliationFederal Research Institute for Animal Healthen_UK
dc.contributor.affiliationChinese Academy of Fishery Sciencesen_UK
dc.contributor.affiliationChinese Academy of Fishery Sciencesen_UK
dc.contributor.affiliationChinese Academy of Fishery Sciencesen_UK
dc.contributor.affiliationChinese Academy of Fishery Sciencesen_UK
dc.contributor.affiliationNational Veterinary Research Instituteen_UK
dc.contributor.affiliationNational Veterinary Research Instituteen_UK
dc.contributor.affiliationFriedrich-Loeffler Instituten_UK
dc.contributor.affiliationFriedrich-Loeffler Instituten_UK
dc.contributor.affiliationANSES - French Agency for Food, Environmental and Occupational Health & Safetyen_UK
dc.contributor.affiliationTechnical University of Denmarken_UK
dc.contributor.affiliationTechnical University of Denmarken_UK
dc.contributor.affiliationGeneReach Biotechnology Corporationen_UK
dc.contributor.affiliationChinese Academy of Fishery Sciencesen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000412593800003en_UK
dc.identifier.scopusid2-s2.0-85030834431en_UK
dc.identifier.wtid533223en_UK
dc.contributor.orcid0000-0002-7692-7756en_UK
dc.date.accepted2017-01-20en_UK
dcterms.dateAccepted2017-01-20en_UK
dc.date.filedepositdate2017-03-22en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorBergmann, Sven M|en_UK
local.rioxx.authorWang, Qing|en_UK
local.rioxx.authorZeng, Weiwei|en_UK
local.rioxx.authorLi, Yingying|en_UK
local.rioxx.authorWang, Yingying|en_UK
local.rioxx.authorMatras, Marek|en_UK
local.rioxx.authorReichert, Michal|en_UK
local.rioxx.authorFichtner, Dieter|en_UK
local.rioxx.authorLenk, Matthias|en_UK
local.rioxx.authorMorin, Thierry|en_UK
local.rioxx.authorOlesen, Niels Jorgen|en_UK
local.rioxx.authorSkall, Helle F|en_UK
local.rioxx.authorLee, Pei-Yu|en_UK
local.rioxx.authorZheng, Shuchen|en_UK
local.rioxx.authorMonaghan, Sean|0000-0002-7692-7756en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-08en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameBergmann_et_al-2017-Journal_of_Fish_Diseases.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0140-7775en_UK
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