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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)
Author(s): Bergmann, Sven M
Wang, Qing
Zeng, Weiwei
Li, Yingying
Wang, Yingying
Matras, Marek
Reichert, Michal
Fichtner, Dieter
Lenk, Matthias
Morin, Thierry
Olesen, Niels Jorgen
Skall, Helle F
Lee, Pei-Yu
Zheng, Shuchen
Monaghan, Sean
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Keywords: carp and koi sera
enzyme-linked immunosorbent assay
koi herpesvirus
koi herpesvirus antibodies
Issue Date: Nov-2017
Date Deposited: 22-Mar-2017
Citation: Bergmann SM, Wang Q, Zeng W, Li Y, Wang Y, Matras M, Reichert M, Fichtner D, Lenk M, Morin T, Olesen NJ, Skall HF, Lee P, Zheng S & Monaghan S (2017) Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA). Journal of Fish Diseases, 40 (11), pp. 1511-1527.
Abstract: Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.
DOI Link: 10.1111/jfd.12621
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Notes: Additional co-authors: S Reiche, W Fuchs, M Kotler, K Way, G Bräuer, K Böttcher, A Kappe, and J Kielpinska
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