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Appears in Collections:Aquaculture Journal Articles
Title: Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay
Author(s): El Wahed, Ahmed Abd
Sanabani, Sabri Saeed
Faye, Oumar
Pessoa, Rodrigo
Patriota, Joao Veras
Giorgi, Ricardo Rodrigues
Patel, Pranav
Bohlken-Fascher, Susanne
Landt, Olfert
Niedrig, Matthias
Zanotto, Paolo M D A
Czerny, Claus-Peter
Sall, Amadou A
Weidmann, Manfred
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Issue Date: 25-Jan-2017
Citation: El Wahed AA, Sanabani SS, Faye O, Pessoa R, Patriota JV, Giorgi RR, Patel P, Bohlken-Fascher S, Landt O, Niedrig M, Zanotto PMDA, Czerny C, Sall AA & Weidmann M (2017) Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay. PLOS Currents: Outbreaks, (1).
Abstract: Background: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-ofcare test is needed to detect the virus, especially at low resource settings.  Methodology/Principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%.  Conclusions/Significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).
DOI Link: 10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319e
Rights: This article is published under a Creative Commons Attribution License, enabling unrestricted distribution and use of the published materials, provided that its authors are properly credited.
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