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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/24855
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dc.contributor.authorEl Wahed, Ahmed Abden_UK
dc.contributor.authorSanabani, Sabri Saeeden_UK
dc.contributor.authorFaye, Oumaren_UK
dc.contributor.authorPessoa, Rodrigoen_UK
dc.contributor.authorPatriota, Joao Verasen_UK
dc.contributor.authorGiorgi, Ricardo Rodriguesen_UK
dc.contributor.authorPatel, Pranaven_UK
dc.contributor.authorBohlken-Fascher, Susanneen_UK
dc.contributor.authorLandt, Olferten_UK
dc.contributor.authorNiedrig, Matthiasen_UK
dc.contributor.authorZanotto, Paolo M D Aen_UK
dc.contributor.authorCzerny, Claus-Peteren_UK
dc.contributor.authorSall, Amadou Aen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.date.accessioned2017-09-15T23:05:36Z-
dc.date.available2017-09-15T23:05:36Z-
dc.date.issued2017-01-25en_UK
dc.identifier.other9en_UK
dc.identifier.urihttp://hdl.handle.net/1893/24855-
dc.description.abstractBackground: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-ofcare test is needed to detect the virus, especially at low resource settings.  Methodology/Principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%.  Conclusions/Significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).en_UK
dc.language.isoenen_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationEl Wahed AA, Sanabani SS, Faye O, Pessoa R, Patriota JV, Giorgi RR, Patel P, Bohlken-Fascher S, Landt O, Niedrig M, Zanotto PMDA, Czerny C, Sall AA & Weidmann M (2017) Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay. PLOS Currents: Outbreaks, (1), Art. No.: 9. https://doi.org/10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319een_UK
dc.rightsThis article is published under a Creative Commons Attribution License, enabling unrestricted distribution and use of the published materials, provided that its authors are properly credited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.titleRapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assayen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319een_UK
dc.identifier.pmid28239513en_UK
dc.citation.jtitlePLOS Currents: Outbreaksen_UK
dc.citation.issn2157-3999en_UK
dc.citation.issue1en_UK
dc.citation.publicationstatusPublisheden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.citation.date25/01/2017en_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationUniversity of Sao Pauloen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationUniversity of Sao Pauloen_UK
dc.contributor.affiliationMunicipal Hospital of Tuparetamaen_UK
dc.contributor.affiliationUniversity of Santo Amaroen_UK
dc.contributor.affiliationTIB MOLBIOL Synthesis Laboratory Ltden_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationTIB MOLBIOL Synthesis Laboratory Ltden_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationUniversity of Sao Pauloen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationInstitut Pasteur de Dakaren_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.wtid537255en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2017-01-25en_UK
dcterms.dateAccepted2017-01-25en_UK
dc.date.filedepositdate2017-01-27en_UK
rioxxterms.apcnot chargeden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorEl Wahed, Ahmed Abd|en_UK
local.rioxx.authorSanabani, Sabri Saeed|en_UK
local.rioxx.authorFaye, Oumar|en_UK
local.rioxx.authorPessoa, Rodrigo|en_UK
local.rioxx.authorPatriota, Joao Veras|en_UK
local.rioxx.authorGiorgi, Ricardo Rodrigues|en_UK
local.rioxx.authorPatel, Pranav|en_UK
local.rioxx.authorBohlken-Fascher, Susanne|en_UK
local.rioxx.authorLandt, Olfert|en_UK
local.rioxx.authorNiedrig, Matthias|en_UK
local.rioxx.authorZanotto, Paolo M D A|en_UK
local.rioxx.authorCzerny, Claus-Peter|en_UK
local.rioxx.authorSall, Amadou A|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2017-01-27en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2017-01-27|en_UK
local.rioxx.filenameAbdElWahed-etal-PLOSCO-2017.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source2157-3999en_UK
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