Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Behavioural fever in zebrafish larvae
Author(s): Rey, Sonia
Moiche Vega, Ana Visila
Boltana, Sebastian
Teles, Mariana
MacKenzie, Simon
Contact Email:
Keywords: Zebrafish larvae
Behavioural fever
Antiviral response
dsRNA challenge
Larval development
Temperature choice
Issue Date: Feb-2017
Date Deposited: 7-Oct-2016
Citation: Rey S, Moiche Vega AV, Boltana S, Teles M & MacKenzie S (2017) Behavioural fever in zebrafish larvae. Developmental and Comparative Immunology, 67, pp. 287-292.
Abstract: Behavioural fever has been reported in different species of mobile ectotherms including the zebrafish,Danio rerio, in response to exogenous pyrogens. In this study we report, to our knowledge for the first time, upon the ontogenic onset of behavioural fever in zebrafish (Danio rerio) larvae. For this, zebrafish larvae (from first feeding to juveniles) were placed in a continuous thermal gradient providing the opportunity to select their preferred temperature. The novel thermal preference aquarium was based upon a continuous vertical column system and allows for non-invasive observation of larvae vertical distribution under isothermal (TRat 28°C) and thermal gradient conditions (TCH: 28-32°C). Larval thermal preference was assessed under both conditions with or without an immersion challenge, in order to detect the onset of the behavioural fever response. Our results defined the onset of the dsRNA induced behavioural fever at 18–20 days post fertilization (dpf). Significant differences were observed in dsRNA challenged larvae, which prefer higher temperatures (1–4°C increase) throughout the experimental period as compared to non-challenged larvae. In parallel we measured the abundance of antiviral transcripts;viperin, gig2, irf7, trim25andMxbmRNAs in dsRNA challenged larvae under both thermal regimes: TRand TCh. Significant increases in the abundance of all measured transcripts were recorded under thermal choice conditions signifying that thermo-coupling and the resultant enhancement of the immune response to dsRNA challenge occurs from 18dpf onwards in the zebrafish. The results are of importance as they identify a key developmental stage where the neuro-immune interface matures in the zebrafish likely providing increased resistance to viral infection.
DOI Link: 10.1016/j.dci.2016.09.008
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Rey S, Moiche Vega AV, Boltana S, Teles M & MacKenzie S (2017) Behavioural fever in zebrafish larvae, Developmental and Comparative Immunology, 67, pp. 287-292. DOI: 10.1016/j.dci.2016.09.008 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL(s):

Files in This Item:
File Description SizeFormat 
Behavioural_fever_in_larvae.pdfFulltext - Accepted Version1.51 MBAdobe PDFView/Open

This item is protected by original copyright

A file in this item is licensed under a Creative Commons License Creative Commons

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.