Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs
Author(s): Grant, Bridie
Picchi, Nicolas
Davie, Andrew
Leclercq, Eric
Migaud, Herve
Contact Email:
Keywords: Cleaner fish
Adhesive eggs
Gum layer
Enzyme alcalase®
Issue Date: 1-Apr-2016
Date Deposited: 25-Aug-2016
Citation: Grant B, Picchi N, Davie A, Leclercq E & Migaud H (2016) Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs. Aquaculture, 456, pp. 44-49.
Abstract: Commercial production of ballan wrasse (Labrus bergylta) as a cleaner fish for the removal of sea lice (Lepeophtheirus salmonis) from farmed salmonids (Salmo salar) has increased due to its proven efficiency. One bottleneck in commercial hatchery production is working with the benthic adhesive eggs, which makes disinfection and incubation of eggs challenging; therefore, this study aimed to find a chemical or enzymatic treatment and process to remove the adhesive gum layer. Naturally spawned eggs were collected from artificial spawning substrates up to 24h post spawning from wild caught broodstock kept in captivity at the Marine Harvest, Machrihanish facility. Four treatments were tested: tannic acid (0.2, 0.1, and 0.05%), sodium sulfite (2, 1, and 0.5%),l-cysteine (2, and 1%), and enzyme alcalase® (4.0, 3.0, 2.0, 1.0, and 0.5%)in vitro. Eggs were exposed for 25min while being continually agitated, and the proportion of “degummed” eggs was counted at the end of each time period. Enzyme alcalase® was the only treatment that proved successful in degumming eggs, with the time to complete degumming (≥96%) inversely related to enzyme concentration. Complete degumming occurred between 15 and 30min for all enzyme alcalase® dose rates. Mean hatch rates for eggs treated with enzyme alcalase ® were not compromised by the treatment and in the highest dose tested were actually found to be higher in treated eggs (78.9±2.4%) than controls (71.3±3.3%). The use of enzyme alcalase® has proven effective in degumming ballan wrasse eggs without affecting hatch rates. However, translation of this method toin situdegumming and thus removal of eggs from spawning substrate on farm remains to be standardised.
DOI Link: 10.1016/j.aquaculture.2016.01.016
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Grant B, Picchi N, Davie A, Leclercq E & Migaud H (2016) Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs, Aquaculture, 456, pp. 44-49. DOI: 10.1016/j.aquaculture.2016.01.016 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL(s):

Files in This Item:
File Description SizeFormat 
Grant et al manuscript revised.pdfFulltext - Accepted Version465.94 kBAdobe PDFView/Open

This item is protected by original copyright

A file in this item is licensed under a Creative Commons License Creative Commons

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.