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dc.contributor.authorGrant, Bridieen_UK
dc.contributor.authorPicchi, Nicolasen_UK
dc.contributor.authorDavie, Andrewen_UK
dc.contributor.authorLeclercq, Ericen_UK
dc.contributor.authorMigaud, Herveen_UK
dc.description.abstractCommercial production of ballan wrasse (Labrus bergylta) as a cleaner fish for the removal of sea lice (Lepeophtheirus salmonis) from farmed salmonids (Salmo salar) has increased due to its proven efficiency. One bottleneck in commercial hatchery production is working with the benthic adhesive eggs, which makes disinfection and incubation of eggs challenging; therefore, this study aimed to find a chemical or enzymatic treatment and process to remove the adhesive gum layer. Naturally spawned eggs were collected from artificial spawning substrates up to 24h post spawning from wild caught broodstock kept in captivity at the Marine Harvest, Machrihanish facility. Four treatments were tested: tannic acid (0.2, 0.1, and 0.05%), sodium sulfite (2, 1, and 0.5%),l-cysteine (2, and 1%), and enzyme alcalase® (4.0, 3.0, 2.0, 1.0, and 0.5%)in vitro. Eggs were exposed for 25min while being continually agitated, and the proportion of “degummed” eggs was counted at the end of each time period. Enzyme alcalase® was the only treatment that proved successful in degumming eggs, with the time to complete degumming (≥96%) inversely related to enzyme concentration. Complete degumming occurred between 15 and 30min for all enzyme alcalase® dose rates. Mean hatch rates for eggs treated with enzyme alcalase ® were not compromised by the treatment and in the highest dose tested were actually found to be higher in treated eggs (78.9±2.4%) than controls (71.3±3.3%). The use of enzyme alcalase® has proven effective in degumming ballan wrasse eggs without affecting hatch rates. However, translation of this method toin situdegumming and thus removal of eggs from spawning substrate on farm remains to be standardised.en_UK
dc.relationGrant B, Picchi N, Davie A, Leclercq E & Migaud H (2016) Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs. Aquaculture, 456, pp. 44-49.
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Grant B, Picchi N, Davie A, Leclercq E & Migaud H (2016) Removal of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggs, Aquaculture, 456, pp. 44-49. DOI: 10.1016/j.aquaculture.2016.01.016 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.subjectCleaner fishen_UK
dc.subjectAdhesive eggsen_UK
dc.subjectGum layeren_UK
dc.subjectEnzyme alcalase®en_UK
dc.titleRemoval of the adhesive gum layer surrounding naturally fertilised ballan wrasse (Labrus bergylta) eggsen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Grant et al manuscript revised.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderTechnology Strategy Boarden_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.relation.funderprojectProduction and Implementation of Farmed Wrasse in the Scottish Salmon Industryen_UK
dc.relation.funderrefApp no 1104_CRD_SAF_SPP_81199en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorGrant, Bridie|en_UK
local.rioxx.authorPicchi, Nicolas|en_UK
local.rioxx.authorDavie, Andrew|0000-0002-9524-618Xen_UK
local.rioxx.authorLeclercq, Eric|en_UK
local.rioxx.authorMigaud, Herve|0000-0002-5404-7512en_UK
local.rioxx.projectApp no 1104_CRD_SAF_SPP_81199|Technology Strategy Board|
local.rioxx.filenameGrant et al manuscript revised.pdfen_UK
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