Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection
Author(s): Yehia, Nahed
Arafa, Abdel-Satar
El Wahed, Ahmed Abd
El-Sanousi, Ahmed A
Weidmann, Manfred
Shalaby, Mohamed A
Contact Email:
Keywords: Avian influenza
Subtype H5N1
Recombinase polymerase amplification assay
Real-time RT-PCR
Issue Date: Oct-2015
Date Deposited: 21-Jan-2016
Citation: Yehia N, Arafa A, El Wahed AA, El-Sanousi AA, Weidmann M & Shalaby MA (2015) Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection. Journal of Virological Methods, 223, pp. 45-49.
Abstract: The 2006 outbreaks of H5N1 avian influenza in Egypt interrupted poultry production and caused staggering economic damage. In addition, H5N1 avian influenza viruses represent a significant threat to public health. Therefore, the rapid detection of H5 viruses is very important in order to control the disease. In this study, a qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of hemagglutinin gene of H5 subtype influenza viruses was developed. The results were compared to the real-time reverse transcription polymerase chain reaction (RT-PCR). Anin vitrotranscribed RNA standard of 970 nucleotides of the hemagglutinin gene was developed and used to determine the assay sensitivity. The developed H5 RT-RPA assay was able to detect one RNA molecule within 7min, while in real-time RT-PCR, at least 90min was required. H5 RT-RPA assay did not detect nucleic acid extracted from H5 negative samples or from other pathogens producing respiratory manifestation in poultry. The clinical performance of the H5 RT-RPA assay was tested in 30 samples collected between 2014 and 2015; the sensitivity of H5 RT-RPA and real-time RT-PCR was 100%. In conclusion, H5 RT-RPA was faster than real-time RT-PCR and easily operable in a portable device. Moreover, it had an equivalent sensitivity and specificity.
DOI Link: 10.1016/j.jviromet.2015.07.011
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Licence URL(s):

Files in This Item:
File Description SizeFormat 
Yehia et al_JVM_2015.pdfFulltext - Published Version598.39 kBAdobe PDFUnder Embargo until 2999-12-29    Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.