Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22809
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dc.contributor.authorYehia, Naheden_UK
dc.contributor.authorArafa, Abdel-Sataren_UK
dc.contributor.authorEl Wahed, Ahmed Abden_UK
dc.contributor.authorEl-Sanousi, Ahmed Aen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorShalaby, Mohamed Aen_UK
dc.date.accessioned2016-05-06T03:49:08Z-
dc.date.available2016-05-06T03:49:08Z-
dc.date.issued2015-10en_UK
dc.identifier.urihttp://hdl.handle.net/1893/22809-
dc.description.abstractThe 2006 outbreaks of H5N1 avian influenza in Egypt interrupted poultry production and caused staggering economic damage. In addition, H5N1 avian influenza viruses represent a significant threat to public health. Therefore, the rapid detection of H5 viruses is very important in order to control the disease. In this study, a qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of hemagglutinin gene of H5 subtype influenza viruses was developed. The results were compared to the real-time reverse transcription polymerase chain reaction (RT-PCR). Anin vitrotranscribed RNA standard of 970 nucleotides of the hemagglutinin gene was developed and used to determine the assay sensitivity. The developed H5 RT-RPA assay was able to detect one RNA molecule within 7min, while in real-time RT-PCR, at least 90min was required. H5 RT-RPA assay did not detect nucleic acid extracted from H5 negative samples or from other pathogens producing respiratory manifestation in poultry. The clinical performance of the H5 RT-RPA assay was tested in 30 samples collected between 2014 and 2015; the sensitivity of H5 RT-RPA and real-time RT-PCR was 100%. In conclusion, H5 RT-RPA was faster than real-time RT-PCR and easily operable in a portable device. Moreover, it had an equivalent sensitivity and specificity.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationYehia N, Arafa A, El Wahed AA, El-Sanousi AA, Weidmann M & Shalaby MA (2015) Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection. Journal of Virological Methods, 223, pp. 45-49. https://doi.org/10.1016/j.jviromet.2015.07.011en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectAvian influenzaen_UK
dc.subjectSubtype H5N1en_UK
dc.subjectRecombinase polymerase amplification assayen_UK
dc.subjectReal-time RT-PCRen_UK
dc.titleDevelopment of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detectionen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-29en_UK
dc.rights.embargoreason[Yehia et al_JVM_2015.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.jviromet.2015.07.011en_UK
dc.identifier.pmid26225482en_UK
dc.citation.jtitleJournal of Virological Methodsen_UK
dc.citation.issn0166-0934en_UK
dc.citation.volume223en_UK
dc.citation.spage45en_UK
dc.citation.epage49en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.citation.date28/07/2015en_UK
dc.contributor.affiliationNational Laboratory for Veterinary Quality Control on Poultry Productionen_UK
dc.contributor.affiliationNational Laboratory for Veterinary Quality Control on Poultry Productionen_UK
dc.contributor.affiliationGerman Primate Centeren_UK
dc.contributor.affiliationCairo Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationCairo Universityen_UK
dc.identifier.isiWOS:000361258700009en_UK
dc.identifier.scopusid2-s2.0-84938718993en_UK
dc.identifier.wtid584638en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2015-07-23en_UK
dcterms.dateAccepted2015-07-23en_UK
dc.date.filedepositdate2016-01-21en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorYehia, Nahed|en_UK
local.rioxx.authorArafa, Abdel-Satar|en_UK
local.rioxx.authorEl Wahed, Ahmed Abd|en_UK
local.rioxx.authorEl-Sanousi, Ahmed A|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorShalaby, Mohamed A|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2999-12-29en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameYehia et al_JVM_2015.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0166-0934en_UK
Appears in Collections:Aquaculture Journal Articles

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