Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/22809
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dc.contributor.authorYehia, Nahed-
dc.contributor.authorArafa, Abdel-Satar-
dc.contributor.authorEl, Wahed Ahmed Abd-
dc.contributor.authorEl-Sanousi, Ahmed A-
dc.contributor.authorWeidmann, Manfred-
dc.contributor.authorShalaby, Mohamed A-
dc.date.accessioned2016-05-06T03:49:08Z-
dc.date.issued2015-10-
dc.identifier.urihttp://hdl.handle.net/1893/22809-
dc.description.abstractThe 2006 outbreaks of H5N1 avian influenza in Egypt interrupted poultry production and caused staggering economic damage. In addition, H5N1 avian influenza viruses represent a significant threat to public health. Therefore, the rapid detection of H5 viruses is very important in order to control the disease. In this study, a qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of hemagglutinin gene of H5 subtype influenza viruses was developed. The results were compared to the real-time reverse transcription polymerase chain reaction (RT-PCR). Anin vitrotranscribed RNA standard of 970 nucleotides of the hemagglutinin gene was developed and used to determine the assay sensitivity. The developed H5 RT-RPA assay was able to detect one RNA molecule within 7min, while in real-time RT-PCR, at least 90min was required. H5 RT-RPA assay did not detect nucleic acid extracted from H5 negative samples or from other pathogens producing respiratory manifestation in poultry. The clinical performance of the H5 RT-RPA assay was tested in 30 samples collected between 2014 and 2015; the sensitivity of H5 RT-RPA and real-time RT-PCR was 100%. In conclusion, H5 RT-RPA was faster than real-time RT-PCR and easily operable in a portable device. Moreover, it had an equivalent sensitivity and specificity.en_UK
dc.language.isoen-
dc.publisherElsevier-
dc.relationYehia N, Arafa A, El Wahed AA, El-Sanousi AA, Weidmann M & Shalaby MA (2015) Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection, Journal of Virological Methods, 223, pp. 45-49.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectAvian influenzaen_UK
dc.subjectSubtype H5N1en_UK
dc.subjectRecombinase polymerase amplification assayen_UK
dc.subjectReal-time RT-PCRen_UK
dc.titleDevelopment of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detectionen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1016/j.jviromet.2015.07.011-
dc.identifier.pmid26225482-
dc.citation.jtitleJournal of Virological Methods-
dc.citation.issn0166-0934-
dc.citation.volume223-
dc.citation.spage45-
dc.citation.epage49-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailm.w.weidmann@stir.ac.uk-
dc.citation.date28/07/2015-
dc.contributor.affiliationNational Laboratory for Veterinary Quality Control on Poultry Production-
dc.contributor.affiliationNational Laboratory for Veterinary Quality Control on Poultry Production-
dc.contributor.affiliationGerman Primate Center-
dc.contributor.affiliationCairo University-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationCairo University-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000361258700009-
Appears in Collections:Aquaculture Journal Articles

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