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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus
Author(s): Callol, Agnes
Reyes-Lopez, Felipe
Roig, Francisco J
Goetz, Giles
Goetz, Frederick W
Amaro, Carmen
MacKenzie, Simon
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Issue Date: 24-Jul-2015
Date Deposited: 17-Sep-2015
Citation: Callol A, Reyes-Lopez F, Roig FJ, Goetz G, Goetz FW, Amaro C & MacKenzie S (2015) An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus. PLoS ONE, 10 (7), Art. No.: e0133328.
Abstract: Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen-associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome. We obtained more than 2x106 reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA13) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA13 were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immune-response is rtxA13-dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue.
DOI Link: 10.1371/journal.pone.0133328
Rights: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication
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