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dc.contributor.authorCallol, Agnesen_UK
dc.contributor.authorReyes-Lopez, Felipeen_UK
dc.contributor.authorRoig, Francisco Jen_UK
dc.contributor.authorGoetz, Gilesen_UK
dc.contributor.authorGoetz, Frederick Wen_UK
dc.contributor.authorAmaro, Carmenen_UK
dc.contributor.authorMacKenzie, Simonen_UK
dc.description.abstractInfectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen-associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome. We obtained more than 2x106 reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA13) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA13 were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immune-response is rtxA13-dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue.en_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationCallol A, Reyes-Lopez F, Roig FJ, Goetz G, Goetz FW, Amaro C & MacKenzie S (2015) An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus. PLoS ONE, 10 (7), Art. No.: e0133328.
dc.rightsThis is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedicationen_UK
dc.titleAn enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificusen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitlePLoS ONEen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversitat Autonoma de Barcelonaen_UK
dc.contributor.affiliationUniversity of Valenciaen_UK
dc.contributor.affiliationNorthwest Fisheries Science Centeren_UK
dc.contributor.affiliationNorthwest Fisheries Science Centeren_UK
dc.contributor.affiliationUniversity of Valenciaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorCallol, Agnes|en_UK
local.rioxx.authorReyes-Lopez, Felipe|en_UK
local.rioxx.authorRoig, Francisco J|en_UK
local.rioxx.authorGoetz, Giles|en_UK
local.rioxx.authorGoetz, Frederick W|en_UK
local.rioxx.authorAmaro, Carmen|en_UK
local.rioxx.authorMacKenzie, Simon|0000-0003-1845-6826en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameCallol et al_Plos One_2015.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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