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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay
Author(s): Xia, Xiaoming
Yu, Yongxin
Weidmann, Manfred
Pan, Yingjie
Yan, Shuling
Wang, Yongjie
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Issue Date: 2014
Date Deposited: 8-Sep-2014
Citation: Xia X, Yu Y, Weidmann M, Pan Y, Yan S & Wang Y (2014) Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay. PLoS ONE, 9 (8), Art. No.: e104667.
Abstract: White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41±0.17 min at 39°C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.
DOI Link: 10.1371/journal.pone.0104667
Rights: © 2014 Xia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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