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dc.contributor.authorXia, Xiaomingen_UK
dc.contributor.authorYu, Yongxinen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorPan, Yingjieen_UK
dc.contributor.authorYan, Shulingen_UK
dc.contributor.authorWang, Yongjieen_UK
dc.description.abstractWhite spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41±0.17 min at 39°C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.en_UK
dc.publisherPublic Library of Scienceen_UK
dc.relationXia X, Yu Y, Weidmann M, Pan Y, Yan S & Wang Y (2014) Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay. PLoS ONE, 9 (8), Art. No.: e104667.
dc.rights© 2014 Xia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_UK
dc.titleRapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assayen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitlePLoS ONEen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationShanghai Ocean Universityen_UK
dc.contributor.affiliationShanghai Ocean Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShanghai Ocean Universityen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationShanghai Ocean Universityen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorXia, Xiaoming|en_UK
local.rioxx.authorYu, Yongxin|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorPan, Yingjie|en_UK
local.rioxx.authorYan, Shuling|en_UK
local.rioxx.authorWang, Yongjie|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenamePlosOne 2014.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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