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STORRE: Stirling Online Research Repository
http://hdl.handle.net/1893/18311| Appears in Collections: | Aquaculture Journal Articles |
| Peer Review Status: | Refereed |
| Title: | Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis |
| Author(s): | Euler, Milena Wang, Yongjie Otto, Peter Tomaso, Herbert Escudero, Raquel Anda, Pedro Hufert, Frank T Weidmann, Manfred |
| Contact Email: | m.w.weidmann@stir.ac.uk |
| Issue Date: | Jul-2012 |
| Date Deposited: | 14-Jan-2014 |
| Citation: | Euler M, Wang Y, Otto P, Tomaso H, Escudero R, Anda P, Hufert FT & Weidmann M (2012) Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis. Journal of Clinical Microbiology, 50 (7), pp. 2234-2238. https://doi.org/10.1128/JCM.06504-11 |
| Abstract: | Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics. |
| DOI Link: | 10.1128/JCM.06504-11 |
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| Licence URL(s): | http://www.rioxx.net/licenses/under-embargo-all-rights-reserved |
| File | Description | Size | Format | |
|---|---|---|---|---|
| JCV 2012.pdf | Fulltext - Published Version | 363.84 kB | Adobe PDF | Under Embargo until 3000-01-01 Request a copy |
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