Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18311
Full metadata record
DC FieldValueLanguage
dc.contributor.authorEuler, Milena-
dc.contributor.authorWang, Yongjie-
dc.contributor.authorOtto, Peter-
dc.contributor.authorTomaso, Herbert-
dc.contributor.authorEscudero, Raquel-
dc.contributor.authorAnda, Pedro-
dc.contributor.authorHufert, Frank T-
dc.contributor.authorWeidmann, Manfred-
dc.date.accessioned2014-11-01T03:43:42Z-
dc.date.issued2012-07-
dc.identifier.urihttp://hdl.handle.net/1893/18311-
dc.description.abstractSeveral real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.en_UK
dc.language.isoen-
dc.publisherAmerican Society for Microbiology-
dc.relationEuler M, Wang Y, Otto P, Tomaso H, Escudero R, Anda P, Hufert FT & Weidmann M (2012) Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis, Journal of Clinical Microbiology, 50 (7), pp. 2234-2238.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.titleRecombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensisen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1128/JCM.06504-11-
dc.citation.jtitleJournal of Clinical Microbiology-
dc.citation.issn0095-1137-
dc.citation.volume50-
dc.citation.issue7-
dc.citation.spage2234-
dc.citation.epage2238-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailm.w.weidmann@stir.ac.uk-
dc.contributor.affiliationUniversity of Gottingen, Georg-August University-
dc.contributor.affiliationUniversity of Gottingen, Georg-August University-
dc.contributor.affiliationFederal Research Institute for Animal Health-
dc.contributor.affiliationFederal Research Institute for Animal Health-
dc.contributor.affiliationCarlos III Health Institute (Instituto de Salud Carlos III)-
dc.contributor.affiliationCarlos III Health Institute (Instituto de Salud Carlos III)-
dc.contributor.affiliationUniversity of Gottingen, Georg-August University-
dc.contributor.affiliationAquaculture-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000307360800011-
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
JCV 2012.pdf363.84 kBAdobe PDFUnder Permanent Embargo    Request a copy


This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.