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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/18311
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dc.contributor.authorEuler, Milenaen_UK
dc.contributor.authorWang, Yongjieen_UK
dc.contributor.authorOtto, Peteren_UK
dc.contributor.authorTomaso, Herberten_UK
dc.contributor.authorEscudero, Raquelen_UK
dc.contributor.authorAnda, Pedroen_UK
dc.contributor.authorHufert, Frank Ten_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.date.accessioned2014-11-01T03:43:42Z-
dc.date.available2014-11-01T03:43:42Zen_UK
dc.date.issued2012-07en_UK
dc.identifier.urihttp://hdl.handle.net/1893/18311-
dc.description.abstractSeveral real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.en_UK
dc.language.isoenen_UK
dc.publisherAmerican Society for Microbiologyen_UK
dc.relationEuler M, Wang Y, Otto P, Tomaso H, Escudero R, Anda P, Hufert FT & Weidmann M (2012) Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis. Journal of Clinical Microbiology, 50 (7), pp. 2234-2238. https://doi.org/10.1128/JCM.06504-11en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.titleRecombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensisen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[JCV 2012.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1128/JCM.06504-11en_UK
dc.citation.jtitleJournal of Clinical Microbiologyen_UK
dc.citation.issn1098-660Xen_UK
dc.citation.issn0095-1137en_UK
dc.citation.volume50en_UK
dc.citation.issue7en_UK
dc.citation.spage2234en_UK
dc.citation.epage2238en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationFederal Research Institute for Animal Healthen_UK
dc.contributor.affiliationFederal Research Institute for Animal Healthen_UK
dc.contributor.affiliationCarlos III Health Institute (Instituto de Salud Carlos III)en_UK
dc.contributor.affiliationCarlos III Health Institute (Instituto de Salud Carlos III)en_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000307360800011en_UK
dc.identifier.scopusid2-s2.0-84862740894en_UK
dc.identifier.wtid674997en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dcterms.dateAccepted2012-07-31en_UK
dc.date.filedepositdate2014-01-14en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorEuler, Milena|en_UK
local.rioxx.authorWang, Yongjie|en_UK
local.rioxx.authorOtto, Peter|en_UK
local.rioxx.authorTomaso, Herbert|en_UK
local.rioxx.authorEscudero, Raquel|en_UK
local.rioxx.authorAnda, Pedro|en_UK
local.rioxx.authorHufert, Frank T|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenameJCV 2012.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0095-1137en_UK
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