Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Performance of Various Commercial Assays for the Detection of Toscana Virus Antibodies
Author(s): Ergunay, Koray
Litzba, Nadine
Lo, Modou Moustapha
Aydogan, Sibel
Saygan, Mehmet B
Us, Durdal
Weidmann, Manfred
Niedrig, Matthias
Contact Email:
Keywords: Diagnosis
Sandfly fever virus
Toscana virus
Issue Date: Jun-2011
Date Deposited: 14-Jan-2014
Citation: Ergunay K, Litzba N, Lo MM, Aydogan S, Saygan MB, Us D, Weidmann M & Niedrig M (2011) Performance of Various Commercial Assays for the Detection of Toscana Virus Antibodies. Vector Borne and Zoonotic Diseases, 11 (6), pp. 781-787.
Abstract: Introduction: Sandfly fever virus (SFV) serotypes sandfly fever Naples virus, sandfly fever Sicilian virus, and sandfly fever Cyprus virus cause febrile diseases, whereas Toscana virus (TOSV) is responsible for aseptic meningoencephalitis. Diagnosis and surveillance of TOSV depend heavily on virus serology, and various commercial assays utilizing various antigen sources and formats have been available. The aim of this study was to perform comparative evaluation of commercially available serological assays for anti-TOSV immunoglobulins. Materials and Methods: A collection of 120 sera from healthy blood donors from an endemic region, previously identified to be reactive for antibodies against various SFV serotypes by indirect immunofluorescence test (IIFT), was reevaluated for IgG/IgM via IIFT, enzyme-linked immunosorbent assay, and an immunoblot assay manufactured by Euroimmun, Diesse, and Mikrogen, respectively. Virus neutralization test (VNT) was performed for 99 sera using standard TOSV, sandfly fever Sicilian virus, and sandfly fever Naples virus strains. Results: A total of 89 samples (74.2%) were reactive for TOSV IgG in at least one of the commercial assays, and 31 samples (31.3%) were reactive in VNT for various SFV serotypes. Average percentage agreements among commercial assays and between VNT and the commercial assays were noted as 57.8% and 62.6%, respectively. No significant correlation between assay results and VNT titers was observed. SFV IgM antibodies were detected in a total of eight samples (6.7%) via IIFT, which were nonreactive in enzyme-linked immunosorbent assay and VNT. Discussion: Commercial diagnostic immunoassays displayed slight to fair agreement for TOSV IgG as assessed via kappa and percentage agreement values. The results could only be confirmed via virus neutralization in a portion of the samples, and overall agreement between the commercial assays and VNT was slight. Commercial assays such as immunoblot can be used in addition to VNT for confirmation of TOSV exposure.
DOI Link: 10.1089/vbz.2010.0224
Rights: This is a copy of an article published in the Vector-Borne and Zoonotic Diseases © 2011 copyright Mary Ann Liebert, Inc.; Vector-Borne and Zoonotic Diseases is available online at:

Files in This Item:
File Description SizeFormat 
Vector Borne and Zoonotic Diseases 2011.pdfFulltext - Published Version144.08 kBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.