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dc.contributor.authorErgunay, Korayen_UK
dc.contributor.authorLitzba, Nadineen_UK
dc.contributor.authorLo, Modou Moustaphaen_UK
dc.contributor.authorAydogan, Sibelen_UK
dc.contributor.authorSaygan, Mehmet Ben_UK
dc.contributor.authorUs, Durdalen_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorNiedrig, Matthiasen_UK
dc.description.abstractIntroduction: Sandfly fever virus (SFV) serotypes sandfly fever Naples virus, sandfly fever Sicilian virus, and sandfly fever Cyprus virus cause febrile diseases, whereas Toscana virus (TOSV) is responsible for aseptic meningoencephalitis. Diagnosis and surveillance of TOSV depend heavily on virus serology, and various commercial assays utilizing various antigen sources and formats have been available. The aim of this study was to perform comparative evaluation of commercially available serological assays for anti-TOSV immunoglobulins. Materials and Methods: A collection of 120 sera from healthy blood donors from an endemic region, previously identified to be reactive for antibodies against various SFV serotypes by indirect immunofluorescence test (IIFT), was reevaluated for IgG/IgM via IIFT, enzyme-linked immunosorbent assay, and an immunoblot assay manufactured by Euroimmun, Diesse, and Mikrogen, respectively. Virus neutralization test (VNT) was performed for 99 sera using standard TOSV, sandfly fever Sicilian virus, and sandfly fever Naples virus strains. Results: A total of 89 samples (74.2%) were reactive for TOSV IgG in at least one of the commercial assays, and 31 samples (31.3%) were reactive in VNT for various SFV serotypes. Average percentage agreements among commercial assays and between VNT and the commercial assays were noted as 57.8% and 62.6%, respectively. No significant correlation between assay results and VNT titers was observed. SFV IgM antibodies were detected in a total of eight samples (6.7%) via IIFT, which were nonreactive in enzyme-linked immunosorbent assay and VNT. Discussion: Commercial diagnostic immunoassays displayed slight to fair agreement for TOSV IgG as assessed via kappa and percentage agreement values. The results could only be confirmed via virus neutralization in a portion of the samples, and overall agreement between the commercial assays and VNT was slight. Commercial assays such as immunoblot can be used in addition to VNT for confirmation of TOSV exposure.en_UK
dc.publisherMary Ann Liebert, Inc.en_UK
dc.relationErgunay K, Litzba N, Lo MM, Aydogan S, Saygan MB, Us D, Weidmann M & Niedrig M (2011) Performance of Various Commercial Assays for the Detection of Toscana Virus Antibodies. Vector Borne and Zoonotic Diseases, 11 (6), pp. 781-787.
dc.rightsThis is a copy of an article published in the Vector-Borne and Zoonotic Diseases © 2011 copyright Mary Ann Liebert, Inc.; Vector-Borne and Zoonotic Diseases is available online at:
dc.subjectSandfly fever virusen_UK
dc.subjectToscana virusen_UK
dc.titlePerformance of Various Commercial Assays for the Detection of Toscana Virus Antibodiesen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleVector-Borne and Zoonotic Diseasesen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationHacettepe Universityen_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
dc.contributor.affiliationGeorg-August University Gottingenen_UK
dc.contributor.affiliationHacettepe Universityen_UK
dc.contributor.affiliationTurkish Red Crescent Societyen_UK
dc.contributor.affiliationHacettepe Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationRobert Koch Instituteen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorErgunay, Koray|en_UK
local.rioxx.authorLitzba, Nadine|en_UK
local.rioxx.authorLo, Modou Moustapha|en_UK
local.rioxx.authorAydogan, Sibel|en_UK
local.rioxx.authorSaygan, Mehmet B|en_UK
local.rioxx.authorUs, Durdal|en_UK
local.rioxx.authorWeidmann, Manfred|0000-0002-7063-7491en_UK
local.rioxx.authorNiedrig, Matthias|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameVector Borne and Zoonotic Diseases 2011.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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