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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens in nervous and non-nervous organs of European seabass (Dicentrarchus labrax) during the course of an experimental challenge
Author(s): Lopez Jimena, Benjamin
Garcia-Rosado, Esther
Thompson, Kimberly
Adams, Alexandra
Infante, Carlos
Borrego, Juan J
Alonso, Maria del Carmen
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Keywords: ELISA
European seabass
Fishes Diseases
European seabass fisheries
Issue Date: Dec-2012
Date Deposited: 12-Jun-2013
Citation: Lopez Jimena B, Garcia-Rosado E, Thompson K, Adams A, Infante C, Borrego JJ & Alonso MdC (2012) Distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens in nervous and non-nervous organs of European seabass (Dicentrarchus labrax) during the course of an experimental challenge. Journal of Veterinary Science, 13 (4), pp. 355-362.
Abstract: The distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens was examined by immunohistochemistry in the nervous and non-nervous organs of juvenile European seabass (Dicentrarchus labrax) during the course of an intramuscular infection. Histological changes resulting from the infection were evaluated from 3 days to 2 months post-infection. The specific antibody response was also studied 2 months post-challenge. Viral proteins were present throughout the experimental period in the retina (inner nuclear layer, ganglion layer, outer limiting membrane, and outer plexiform layer), brain (cerebellum and tectum opticum), and liver (hepatocytes and endothelial cells). These proteins were also observed in the renal tubular cells, white pulp of spleen, and in fibroblasts and cartilage of caudal fin. This is the first report of RGNNV proteins appearing in these organs, where the immunostaining was only detected at certain sampling times after the onset of mortality. Brain and retina of virus-exposed fish showed high levels of vacuolation, while accumulation of fat vacuoles was observed in the liver. RGNNV infection also induced a specific antibody response as measured by an ELISA. In summary, this is the first study demonstrating the presence of viral proteins in cells of caudal fin, kidney and spleen of European seabass.
DOI Link: 10.4142/jvs.2012.13.4.355
Rights: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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