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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish
Author(s): Poobalane, Saravanane
Thompson, Kimberly
Ardo, Laszlo
Verjan, Noel
Han, Hyun-Ja
Jeney, Galina
Hirono, Ikuo
Aoki, Takashi
Adams, Alexandra
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Keywords: Aeromonas hydrophila
Recombinant S-layer protein vaccine
Efficacy testing
Common carp
Issue Date: Apr-2010
Citation: Poobalane S, Thompson K, Ardo L, Verjan N, Han H, Jeney G, Hirono I, Aoki T & Adams A (2010) Production and efficacy of an Aeromonas hydrophila recombinant S-layer protein vaccine for fish, Vaccine, 28 (20), pp. 3540-3547.
Abstract: A recombinant protein for the S-layer protein of Aeromonas hydrophila was produced and its ability to protect common carp Cyprinus carpio L. against six virulent isolates of A. hydrophila was assessed. A group of 120 carp (30-40 g) were vaccinated intra-peritoneally with 0.1 ml of adjuvanted vaccine (30 μg protein per fish). Another group of 120 carp were injected with 0.1 ml of PBS-adjuvant mixture to serve as controls. Twenty fish from each group were challenged with each one of six virulent isolates of A. hydrophila 35 days post-vaccination. The fish were maintained in 12 separate tanks before terminating the experiment at 16 days post-challenge. The relative percentage survival (RPS) for the six isolates of A. hydrophila ranged from 56 to 87%. The difference in survival rate of fish challenged with four of the isolates was statistically significant in vaccinated fish compared to control fish, when analysed using a Chi-square test. The results of the study suggest that the recombinant S-layer protein of A. hydrophila could be useful as a vaccine antigen to protect fish against different isolates of this pathogenic bacterium.
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