Please use this identifier to cite or link to this item:
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Alternative cell line for the isolation of salmonid alphavirus-1
Author(s): Herath, Tharangani
Costa, Janina Z
Thompson, Kimberly
Adams, Alexandra
Richards, Randolph
Contact Email:
Keywords: Cell culture
Immune response Fishes
Atlantic salmon
Issue Date: 2009
Date Deposited: 12-Jun-2013
Citation: Herath T, Costa JZ, Thompson K, Adams A & Richards R (2009) Alternative cell line for the isolation of salmonid alphavirus-1. Icelandic Agricultural Sciences, 22, pp. 19-27.$FILE/IAS%20Alternative%20cell%20line.pdf
Abstract: Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aquaculture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland; several established cell lines have since been tested for viral growth, although the ability of these cell lines to support primary virus isolation has not being examined. In the present study, CHSE-214, Chum salmon heart -1 (CHH-1) and Salmon head kidney -1 (SHK-1) cell lines were evaluated for isolation of SAV-1 from kidney samples of experimentally infected Atlantic salmon (Salmo salar). The presence of infection in these samples was confirmed both by cell culture and reverse transcription polymerase chain reaction (RT-PCR). Homogenates of kidney from fish 3 days post-infection (p.i.) were inoculated onto the three cell lines and the development of a cytopathic effect (CPE) recorded. The CHH-1 cells produced a rapid CPE from Day 6 p.i., while the CHSE-214 cells showed the presence of a CPE from Day 10 p.i. In comparison, a CPE developed much later in the SHK-1 cells, from Day 20 p.i. The virus was successfully isolated on all three cell lines in subsequent passages, indicating that CHSE-214, CHH-1, and SHK-1 cells can be used for the isolation and culture of SAV-1. The CHH-1 cell line, however, has proven the most useful, since the CPE developed the quickest in this cell line.
Rights: The publisher has granted permission for use of this work in this Repository. Published in Icelandic Agricultural Sciences, 22, pp.19-27, 2009 by a Consortium of universities and research institutions in Iceland:$FILE/IAS%20Alternative%20cell%20line.pdf

Files in This Item:
File Description SizeFormat 
ias alternative cell line.pdfFulltext - Published Version381.98 kBAdobe PDFView/Open

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.