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dc.contributor.authorHerath, Tharanganien_UK
dc.contributor.authorCosta, Janina Zen_UK
dc.contributor.authorThompson, Kimberlyen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorRichards, Randolphen_UK
dc.description.abstractSalmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aquaculture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland; several established cell lines have since been tested for viral growth, although the ability of these cell lines to support primary virus isolation has not being examined. In the present study, CHSE-214, Chum salmon heart -1 (CHH-1) and Salmon head kidney -1 (SHK-1) cell lines were evaluated for isolation of SAV-1 from kidney samples of experimentally infected Atlantic salmon (Salmo salar). The presence of infection in these samples was confirmed both by cell culture and reverse transcription polymerase chain reaction (RT-PCR). Homogenates of kidney from fish 3 days post-infection (p.i.) were inoculated onto the three cell lines and the development of a cytopathic effect (CPE) recorded. The CHH-1 cells produced a rapid CPE from Day 6 p.i., while the CHSE-214 cells showed the presence of a CPE from Day 10 p.i. In comparison, a CPE developed much later in the SHK-1 cells, from Day 20 p.i. The virus was successfully isolated on all three cell lines in subsequent passages, indicating that CHSE-214, CHH-1, and SHK-1 cells can be used for the isolation and culture of SAV-1. The CHH-1 cell line, however, has proven the most useful, since the CPE developed the quickest in this cell line.en_UK
dc.publisherConsortium of universities and research institutions in Icelanden_UK
dc.relationHerath T, Costa JZ, Thompson K, Adams A & Richards R (2009) Alternative cell line for the isolation of salmonid alphavirus-1. Icelandic Agricultural Sciences, 22, pp. 19-27.$FILE/IAS%20Alternative%20cell%20line.pdfen_UK
dc.rightsThe publisher has granted permission for use of this work in this Repository. Published in Icelandic Agricultural Sciences, 22, pp.19-27, 2009 by a Consortium of universities and research institutions in Iceland:$FILE/IAS%20Alternative%20cell%20line.pdfen_UK
dc.subjectCell cultureen_UK
dc.subjectImmune response Fishesen_UK
dc.subjectAtlantic salmonen_UK
dc.titleAlternative cell line for the isolation of salmonid alphavirus-1en_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleIcelandic Agricultural Sciencesen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Stirlingen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorHerath, Tharangani|en_UK
local.rioxx.authorCosta, Janina Z|en_UK
local.rioxx.authorThompson, Kimberly|en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.authorRichards, Randolph|en_UK
local.rioxx.projectInternal Project|University of Stirling|
local.rioxx.filenameias alternative cell line.pdfen_UK
Appears in Collections:Aquaculture Journal Articles

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