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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Teleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genes
Author(s): Martins, Dulce Alves
Rocha, Filipa
Martinez-Rodriguez, Gonzalo
Bell, J Gordon
Morais, Sofia
Castanheira, Filipa
Bandarra, Narcisa
Coutinho, Joana
Yufera, Manuel
Conceicao, Luis E C
Keywords: PUFA
Sparus aurata
Gene expression
Issue Date: 14-Sep-2012
Date Deposited: 26-Nov-2012
Citation: Martins DA, Rocha F, Martinez-Rodriguez G, Bell JG, Morais S, Castanheira F, Bandarra N, Coutinho J, Yufera M & Conceicao LEC (2012) Teleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genes. British Journal of Nutrition, 108 (5), pp. 864-874.
Abstract: Dietary fatty acid supply can affect stress response in fish during early development. Although knowledge on the mechanisms involved in fatty acid regulation of stress tolerance is scarce, it has often been hypothesised that eicosanoid profiles can influence cortisol production. Genomic cortisol actions are mediated by cytosolic receptors which may respond to cellular fatty acid signalling. An experiment was designed to test the effects of feeding gilthead sea-bream larvae with four microdiets, containing graded arachidonic acid (ARA) levels (0·4, 0·8, 1·5 and 3·0 %), on the expression of genes involved in stress response (steroidogenic acute regulatory protein, glucocorticoid receptor and phosphoenolpyruvate carboxykinase), lipid and, particularly, eicosanoid metabolism (hormone-sensitive lipase, PPARα, phospholipase A2, cyclo-oxygenase-2 and 5-lipoxygenase), as determined by real-time quantitative PCR. Fish fatty acid phenotypes reflected dietary fatty acid profiles. Growth performance, survival after acute stress and similar whole-body basal cortisol levels suggested that sea-bream larvae could tolerate a wide range of dietary ARA levels. Transcription of all genes analysed was significantly reduced at dietary ARA levels above 0·4 %. Nonetheless, despite practical suppression of phospholipase A2 transcription, higher leukotriene B4 levels were detected in larvae fed 3·0 % ARA, whereas a similar trend was observed regarding PGE2 production. The present study demonstrates that adaptation to a wide range of dietary ARA levels in gilthead sea-bream larvae involves the modulation of the expression of genes related to eicosanoid synthesis, lipid metabolism and stress response. The roles of ARA, other polyunsaturates and eicosanoids as signals in this process are discussed.
DOI Link: 10.1017/S0007114511006143
Rights: Publisher policy allows this work to be made available in this repository. Published in British Journal of Nutrition, Volume 108, Issue 05, September 2012, pp 864-874 copyright Cambridge University Press. The original publication is available at DOI:

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